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Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV)
The spike (S) glycoprotein is thought to play a complex and central role in the biology and pathogenesis of SARS coronavirus infection. In this study, a recombinant protein (rS268, corresponding to residues 268–1255 of SARS-CoV S protein) was expressed in Escherichia coli and was purified to near ho...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089214/ https://www.ncbi.nlm.nih.gov/pubmed/16132115 http://dx.doi.org/10.1007/s11373-005-9004-3 |
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author | Lai, Szu-Chia Chong, Pele Choi-Sing Yeh, Chia-Tsui Liu, Levent Shih-Jen Jan, Jia-Tsrong Chi, Hsiang-Yun Liu, Hwan-Wun Chen, Ann Wang, Yeau-Ching |
author_facet | Lai, Szu-Chia Chong, Pele Choi-Sing Yeh, Chia-Tsui Liu, Levent Shih-Jen Jan, Jia-Tsrong Chi, Hsiang-Yun Liu, Hwan-Wun Chen, Ann Wang, Yeau-Ching |
author_sort | Lai, Szu-Chia |
collection | PubMed |
description | The spike (S) glycoprotein is thought to play a complex and central role in the biology and pathogenesis of SARS coronavirus infection. In this study, a recombinant protein (rS268, corresponding to residues 268–1255 of SARS-CoV S protein) was expressed in Escherichia coli and was purified to near homogeneity. After immunization with rS268, S protein-specific BALB/c antisera and mAbs were induced and confirmed using ELISA, Western blot and IFA. Several BALB/c mAbs were found to be effectively to neutralize the infection of Vero E6 cells by SARS-CoV in a dose-dependent manner. Systematic epitope mapping showed that all these neutralizing mAbs recognized a 15-residues peptide (CB-119) corresponding to residues 1143–1157 (SPDVDLGDISGINAS) that was located to the second heptad repeat (HR2) region of the SARS-CoV spike protein. The peptide CB-119 could specifically inhibit the interaction of neutralizing mAbs and spike protein in a dose-dependent manner. Further, neutralizing mAbs, but not control mAbs, could specifically interact with CB-119 in a dose-dependent manner. Results implicated that the second heptad repeat region of spike protein could be a good target for vaccine development against SARS-CoV. |
format | Online Article Text |
id | pubmed-7089214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-70892142020-03-23 Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) Lai, Szu-Chia Chong, Pele Choi-Sing Yeh, Chia-Tsui Liu, Levent Shih-Jen Jan, Jia-Tsrong Chi, Hsiang-Yun Liu, Hwan-Wun Chen, Ann Wang, Yeau-Ching J Biomed Sci Article The spike (S) glycoprotein is thought to play a complex and central role in the biology and pathogenesis of SARS coronavirus infection. In this study, a recombinant protein (rS268, corresponding to residues 268–1255 of SARS-CoV S protein) was expressed in Escherichia coli and was purified to near homogeneity. After immunization with rS268, S protein-specific BALB/c antisera and mAbs were induced and confirmed using ELISA, Western blot and IFA. Several BALB/c mAbs were found to be effectively to neutralize the infection of Vero E6 cells by SARS-CoV in a dose-dependent manner. Systematic epitope mapping showed that all these neutralizing mAbs recognized a 15-residues peptide (CB-119) corresponding to residues 1143–1157 (SPDVDLGDISGINAS) that was located to the second heptad repeat (HR2) region of the SARS-CoV spike protein. The peptide CB-119 could specifically inhibit the interaction of neutralizing mAbs and spike protein in a dose-dependent manner. Further, neutralizing mAbs, but not control mAbs, could specifically interact with CB-119 in a dose-dependent manner. Results implicated that the second heptad repeat region of spike protein could be a good target for vaccine development against SARS-CoV. Springer Netherlands 2005-08-19 /pmc/articles/PMC7089214/ /pubmed/16132115 http://dx.doi.org/10.1007/s11373-005-9004-3 Text en © National Science Council Taipei 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Lai, Szu-Chia Chong, Pele Choi-Sing Yeh, Chia-Tsui Liu, Levent Shih-Jen Jan, Jia-Tsrong Chi, Hsiang-Yun Liu, Hwan-Wun Chen, Ann Wang, Yeau-Ching Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title | Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title_full | Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title_fullStr | Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title_full_unstemmed | Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title_short | Characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein HR2 region of severe acute respiratory syndrome coronavirus (SARS-CoV) |
title_sort | characterization of neutralizing monoclonal antibodies recognizing a 15-residues epitope on the spike protein hr2 region of severe acute respiratory syndrome coronavirus (sars-cov) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089214/ https://www.ncbi.nlm.nih.gov/pubmed/16132115 http://dx.doi.org/10.1007/s11373-005-9004-3 |
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