Sequence determination of the extreme 5′ end of equine arteritis virus leader region

The extreme 5′ end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5′ RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5′ end of the EAV published genomic sequence. A common feature among the...

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Detalles Bibliográficos
Autores principales: Kheyar, Ali, St-Laurent, Gilles, Archambault, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 1996
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089268/
https://www.ncbi.nlm.nih.gov/pubmed/8883367
http://dx.doi.org/10.1007/BF00284650
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author Kheyar, Ali
St-Laurent, Gilles
Archambault, Denis
author_facet Kheyar, Ali
St-Laurent, Gilles
Archambault, Denis
author_sort Kheyar, Ali
collection PubMed
description The extreme 5′ end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5′ RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5′ end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. The role of this putative intraleader ORF has yet to be determined.
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spelling pubmed-70892682020-03-23 Sequence determination of the extreme 5′ end of equine arteritis virus leader region Kheyar, Ali St-Laurent, Gilles Archambault, Denis Virus Genes Article The extreme 5′ end of the leader sequence of four equine arteritis virus (EAV) strains was obtained by using rapid amplification of cDNA end method (5′ RACE), and sequenced. Seventeen more nucleotides were added upstream of the 5′ end of the EAV published genomic sequence. A common feature among the analyzed EAV isolates was the presence of an AUG start codon within the added sequence and the appearance of an intraleader open reading frame (ORF) of 111 nucleotides which was predicted to encode a peptide of 37 amino acids. The role of this putative intraleader ORF has yet to be determined. Kluwer Academic Publishers 1996 /pmc/articles/PMC7089268/ /pubmed/8883367 http://dx.doi.org/10.1007/BF00284650 Text en © Kluwer Academic Publishers 1996 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Kheyar, Ali
St-Laurent, Gilles
Archambault, Denis
Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title_full Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title_fullStr Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title_full_unstemmed Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title_short Sequence determination of the extreme 5′ end of equine arteritis virus leader region
title_sort sequence determination of the extreme 5′ end of equine arteritis virus leader region
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089268/
https://www.ncbi.nlm.nih.gov/pubmed/8883367
http://dx.doi.org/10.1007/BF00284650
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