Cargando…
Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region
Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS). Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417–547 of S protein. However, this region has no homology with the newly separated SAR...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Science in China Press
2003
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089281/ https://www.ncbi.nlm.nih.gov/pubmed/32214699 http://dx.doi.org/10.1007/BF03185764 |
_version_ | 1783509701971935232 |
---|---|
author | Lu, Yun Chen, Yinghua |
author_facet | Lu, Yun Chen, Yinghua |
author_sort | Lu, Yun |
collection | PubMed |
description | Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS). Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417–547 of S protein. However, this region has no homology with the newly separated SARS-associated virus (Hong Kong isolate CUHK-W1). Then we analyzed the phylogenesis of S1 subunit of the coronavirus spike protein (SARS-associated virus, Hong Kong isolate CUHK-W1). Interestingly, the highest homology between murine hepatitis virus (MHV) and SARS-associated coronavirus was found. And the important sites (aa62–65 and aa214–216) on the spike protein of MHV with receptor-binding capacity were highly conservative in comparison with the newly separated SARS-associated virus (the corresponding sites are aa51–54 and aal95–197). These results from bioinformatics analysis might help us to study the receptor-binding sites of SARS-associated virus and the mechanism of the virus entry into the target cell, and design antiviral drugs and potent vaccines. |
format | Online Article Text |
id | pubmed-7089281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Science in China Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70892812020-03-23 Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region Lu, Yun Chen, Yinghua Chin Sci Bull Reports Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS). Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417–547 of S protein. However, this region has no homology with the newly separated SARS-associated virus (Hong Kong isolate CUHK-W1). Then we analyzed the phylogenesis of S1 subunit of the coronavirus spike protein (SARS-associated virus, Hong Kong isolate CUHK-W1). Interestingly, the highest homology between murine hepatitis virus (MHV) and SARS-associated coronavirus was found. And the important sites (aa62–65 and aa214–216) on the spike protein of MHV with receptor-binding capacity were highly conservative in comparison with the newly separated SARS-associated virus (the corresponding sites are aa51–54 and aal95–197). These results from bioinformatics analysis might help us to study the receptor-binding sites of SARS-associated virus and the mechanism of the virus entry into the target cell, and design antiviral drugs and potent vaccines. Science in China Press 2003 /pmc/articles/PMC7089281/ /pubmed/32214699 http://dx.doi.org/10.1007/BF03185764 Text en © Science in China Press 2003 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Reports Lu, Yun Chen, Yinghua Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title | Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title_full | Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title_fullStr | Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title_full_unstemmed | Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title_short | Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
title_sort | spike protein homology between the sars-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089281/ https://www.ncbi.nlm.nih.gov/pubmed/32214699 http://dx.doi.org/10.1007/BF03185764 |
work_keys_str_mv | AT luyun spikeproteinhomologybetweenthesarsassociatedvirusandmurinehepatitisvirusimpliesexistenceofaputativereceptorbindingregion AT chenyinghua spikeproteinhomologybetweenthesarsassociatedvirusandmurinehepatitisvirusimpliesexistenceofaputativereceptorbindingregion |