Expression and purification of SARS coronavirus membrane protein

To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed...

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Detalles Bibliográficos
Autores principales: Wuxing, Dai, Mingjun, Lei, Shaoting, Wu, Zhihao, Chen, Liang, Liang, Hujrong, Pan, Li, Qin, Shitong, Gao, Shishan, Yuan, Renli, Zhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Huazhong University of Science and Technology 2004
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089427/
https://www.ncbi.nlm.nih.gov/pubmed/15641679
http://dx.doi.org/10.1007/BF02831095

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