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Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus
In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed for detection of porcine epidemic diarrhea virus (PEDV). Six primers were designed to amplify the nucleocapsid (N) gene of PEDV. The optimization, sensitivity, and specificity of the RT-LAMP were in...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089436/ https://www.ncbi.nlm.nih.gov/pubmed/21286798 http://dx.doi.org/10.1007/s11262-011-0570-3 |