Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species

A sheep kidney-derived cell line, FLK-N3, was successfully established after serial (>100) passages. Persistent infection of this cell line with viruses and mycoplasma was not detected. The cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porc...

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Autores principales: Matsuura, Katsuyoshi, Inoshima, Yasuo, Kameyama, Ken-ichiro, Murakami, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089453/
https://www.ncbi.nlm.nih.gov/pubmed/21695582
http://dx.doi.org/10.1007/s11626-011-9434-3
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author Matsuura, Katsuyoshi
Inoshima, Yasuo
Kameyama, Ken-ichiro
Murakami, Kenji
author_facet Matsuura, Katsuyoshi
Inoshima, Yasuo
Kameyama, Ken-ichiro
Murakami, Kenji
author_sort Matsuura, Katsuyoshi
collection PubMed
description A sheep kidney-derived cell line, FLK-N3, was successfully established after serial (>100) passages. Persistent infection of this cell line with viruses and mycoplasma was not detected. The cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bovine enterovirus, suid herpesvirus 1, and porcine enterovirus. These results suggest that the FLK-N3 cell line could be useful for isolation and propagation of viruses that affect cloven-hoofed animals.
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spelling pubmed-70894532020-03-23 Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species Matsuura, Katsuyoshi Inoshima, Yasuo Kameyama, Ken-ichiro Murakami, Kenji In Vitro Cell Dev Biol Anim Report A sheep kidney-derived cell line, FLK-N3, was successfully established after serial (>100) passages. Persistent infection of this cell line with viruses and mycoplasma was not detected. The cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bovine enterovirus, suid herpesvirus 1, and porcine enterovirus. These results suggest that the FLK-N3 cell line could be useful for isolation and propagation of viruses that affect cloven-hoofed animals. Springer-Verlag 2011-06-22 2011 /pmc/articles/PMC7089453/ /pubmed/21695582 http://dx.doi.org/10.1007/s11626-011-9434-3 Text en © The Society for In Vitro Biology 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Report
Matsuura, Katsuyoshi
Inoshima, Yasuo
Kameyama, Ken-ichiro
Murakami, Kenji
Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title_full Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title_fullStr Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title_full_unstemmed Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title_short Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
title_sort establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089453/
https://www.ncbi.nlm.nih.gov/pubmed/21695582
http://dx.doi.org/10.1007/s11626-011-9434-3
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