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Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells

Many genes encoding growth factors, receptors, and transcription factors are induced by the epithelial-mesenchymal interaction during tooth development. Recently, numerous functions of microRNAs (miRNAs) are reportedly involved in organogenesis and disease. miRNAs regulate gene expression by inhibit...

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Autores principales: Nakamura, Tomoaki, Iwamoto, Tsutomu, Nakamura, Hannah M., Shindo, Yuki, Saito, Kan, Yamada, Aya, Yamada, Yoshihiko, Fukumoto, Satoshi, Nakamura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089876/
https://www.ncbi.nlm.nih.gov/pubmed/32258035
http://dx.doi.org/10.3389/fcell.2020.00156
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author Nakamura, Tomoaki
Iwamoto, Tsutomu
Nakamura, Hannah M.
Shindo, Yuki
Saito, Kan
Yamada, Aya
Yamada, Yoshihiko
Fukumoto, Satoshi
Nakamura, Takashi
author_facet Nakamura, Tomoaki
Iwamoto, Tsutomu
Nakamura, Hannah M.
Shindo, Yuki
Saito, Kan
Yamada, Aya
Yamada, Yoshihiko
Fukumoto, Satoshi
Nakamura, Takashi
author_sort Nakamura, Tomoaki
collection PubMed
description Many genes encoding growth factors, receptors, and transcription factors are induced by the epithelial-mesenchymal interaction during tooth development. Recently, numerous functions of microRNAs (miRNAs) are reportedly involved in organogenesis and disease. miRNAs regulate gene expression by inhibiting translation and destabilizing mRNAs. However, the expression and function of miRNAs in tooth development remain poorly understood. This study aimed to analyze the expression of miRNAs produced during tooth development using a microarray system to clarify the role of miRNAs in dental development. miR-1 showed a unique expression pattern in the developing tooth. miR-1 expression in the tooth germ peaked on embryonic day 16.5, decreasing gradually on postnatal days 1 and 3. An in situ hybridization assay revealed that miR-1 is expressed at the cervical loop of the dental epithelium. The expression of miR-1 and connexin (Cx) 43, a target of miR-1, were inversely correlated both in vitro and in vivo. Knockdown of miR-1 induced the expression of Cx43 in dental epithelial cells. Interestingly, cells with miR-1 downregulation proliferated slower than the control cells. Immunocytochemistry revealed that Cx43 in cells with miR-1 knockdown formed both cell-cell gap junctions and hemichannels at the plasma membrane. Furthermore, the rate of ATP release was higher in cells with miR-1 knockdown than in control cells. Furthermore, Cx43 downregulation in developing molars was observed in Epiprofin-knockout mice, along with the induction of miR-1 expression. These results suggest that the expression pattern of Cx43 is modulated by miR-1 to control cell proliferation activity during dental epithelial cell differentiation.
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spelling pubmed-70898762020-03-31 Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells Nakamura, Tomoaki Iwamoto, Tsutomu Nakamura, Hannah M. Shindo, Yuki Saito, Kan Yamada, Aya Yamada, Yoshihiko Fukumoto, Satoshi Nakamura, Takashi Front Cell Dev Biol Cell and Developmental Biology Many genes encoding growth factors, receptors, and transcription factors are induced by the epithelial-mesenchymal interaction during tooth development. Recently, numerous functions of microRNAs (miRNAs) are reportedly involved in organogenesis and disease. miRNAs regulate gene expression by inhibiting translation and destabilizing mRNAs. However, the expression and function of miRNAs in tooth development remain poorly understood. This study aimed to analyze the expression of miRNAs produced during tooth development using a microarray system to clarify the role of miRNAs in dental development. miR-1 showed a unique expression pattern in the developing tooth. miR-1 expression in the tooth germ peaked on embryonic day 16.5, decreasing gradually on postnatal days 1 and 3. An in situ hybridization assay revealed that miR-1 is expressed at the cervical loop of the dental epithelium. The expression of miR-1 and connexin (Cx) 43, a target of miR-1, were inversely correlated both in vitro and in vivo. Knockdown of miR-1 induced the expression of Cx43 in dental epithelial cells. Interestingly, cells with miR-1 downregulation proliferated slower than the control cells. Immunocytochemistry revealed that Cx43 in cells with miR-1 knockdown formed both cell-cell gap junctions and hemichannels at the plasma membrane. Furthermore, the rate of ATP release was higher in cells with miR-1 knockdown than in control cells. Furthermore, Cx43 downregulation in developing molars was observed in Epiprofin-knockout mice, along with the induction of miR-1 expression. These results suggest that the expression pattern of Cx43 is modulated by miR-1 to control cell proliferation activity during dental epithelial cell differentiation. Frontiers Media S.A. 2020-03-17 /pmc/articles/PMC7089876/ /pubmed/32258035 http://dx.doi.org/10.3389/fcell.2020.00156 Text en Copyright © 2020 Nakamura, Iwamoto, Nakamura, Shindo, Saito, Yamada, Yamada, Fukumoto and Nakamura. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Nakamura, Tomoaki
Iwamoto, Tsutomu
Nakamura, Hannah M.
Shindo, Yuki
Saito, Kan
Yamada, Aya
Yamada, Yoshihiko
Fukumoto, Satoshi
Nakamura, Takashi
Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title_full Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title_fullStr Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title_full_unstemmed Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title_short Regulation of miR-1-Mediated Connexin 43 Expression and Cell Proliferation in Dental Epithelial Cells
title_sort regulation of mir-1-mediated connexin 43 expression and cell proliferation in dental epithelial cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089876/
https://www.ncbi.nlm.nih.gov/pubmed/32258035
http://dx.doi.org/10.3389/fcell.2020.00156
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