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Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.

Polyploidization often leads to “transcriptome shock,” and is considered an important factor in evolution of species. Analysis of the cell cycle, which is associated with survival in polyploidy, has proved useful in investigating polyploidization. Here, we used mRNA sequencing to investigate global...

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Autores principales: Ren, Li, Lu, Jiahao, Fan, Yunpeng, Hu, Yibo, Li, Jiaming, Xiao, Yamei, Liu, Shaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089929/
https://www.ncbi.nlm.nih.gov/pubmed/32256518
http://dx.doi.org/10.3389/fgene.2020.00203
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author Ren, Li
Lu, Jiahao
Fan, Yunpeng
Hu, Yibo
Li, Jiaming
Xiao, Yamei
Liu, Shaojun
author_facet Ren, Li
Lu, Jiahao
Fan, Yunpeng
Hu, Yibo
Li, Jiaming
Xiao, Yamei
Liu, Shaojun
author_sort Ren, Li
collection PubMed
description Polyploidization often leads to “transcriptome shock,” and is considered an important factor in evolution of species. Analysis of the cell cycle, which is associated with survival in polyploidy, has proved useful in investigating polyploidization. Here, we used mRNA sequencing to investigate global expression in vitro (in cultured cells) and in vivo (in fin and liver tissues) in both the diploid and tetraploid Carassius auratus red var.. Differential expression (DE) of genes in diploid (7482, 36.0%) and tetraploid (3787, 18.2%) states suggested that in vitro and in vivo conditions dramatically change mRNA expression levels. However, of the 20,771 total shared expressed genes, 18,050 (87.0%), including 17,905 (86.2%) non-differentially expressed genes (DEGs) and 145 (0.7%) DEGs between diploids and tetraploids, showed the same expression trends in both cultured cells and liver tissues. Of the DEGs, four of seven genes in the cell cycle pathway had the same expression trends (upregulated in diploids and tetraploids) in both cultured cells and liver tissues. Quantitative PCR analysis confirmed the same expression trends in the nine DEGs associated with regulation of the cell cycle. This research on common characteristics between diploids and tetraploids provides insights into the potential molecular regulatory mechanisms of polyploidization. The steady changes that occur between diploids and tetraploids in vitro and in vivo show the potential value of studying polyploidy processes using cultured cell lines, especially with respect to cell cycle regulation.
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spelling pubmed-70899292020-03-31 Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var. Ren, Li Lu, Jiahao Fan, Yunpeng Hu, Yibo Li, Jiaming Xiao, Yamei Liu, Shaojun Front Genet Genetics Polyploidization often leads to “transcriptome shock,” and is considered an important factor in evolution of species. Analysis of the cell cycle, which is associated with survival in polyploidy, has proved useful in investigating polyploidization. Here, we used mRNA sequencing to investigate global expression in vitro (in cultured cells) and in vivo (in fin and liver tissues) in both the diploid and tetraploid Carassius auratus red var.. Differential expression (DE) of genes in diploid (7482, 36.0%) and tetraploid (3787, 18.2%) states suggested that in vitro and in vivo conditions dramatically change mRNA expression levels. However, of the 20,771 total shared expressed genes, 18,050 (87.0%), including 17,905 (86.2%) non-differentially expressed genes (DEGs) and 145 (0.7%) DEGs between diploids and tetraploids, showed the same expression trends in both cultured cells and liver tissues. Of the DEGs, four of seven genes in the cell cycle pathway had the same expression trends (upregulated in diploids and tetraploids) in both cultured cells and liver tissues. Quantitative PCR analysis confirmed the same expression trends in the nine DEGs associated with regulation of the cell cycle. This research on common characteristics between diploids and tetraploids provides insights into the potential molecular regulatory mechanisms of polyploidization. The steady changes that occur between diploids and tetraploids in vitro and in vivo show the potential value of studying polyploidy processes using cultured cell lines, especially with respect to cell cycle regulation. Frontiers Media S.A. 2020-03-17 /pmc/articles/PMC7089929/ /pubmed/32256518 http://dx.doi.org/10.3389/fgene.2020.00203 Text en Copyright © 2020 Ren, Lu, Fan, Hu, Li, Xiao and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ren, Li
Lu, Jiahao
Fan, Yunpeng
Hu, Yibo
Li, Jiaming
Xiao, Yamei
Liu, Shaojun
Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title_full Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title_fullStr Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title_full_unstemmed Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title_short Expression Profile Analysis of the Cell Cycle in Diploid and Tetraploid Carassius auratus red var.
title_sort expression profile analysis of the cell cycle in diploid and tetraploid carassius auratus red var.
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089929/
https://www.ncbi.nlm.nih.gov/pubmed/32256518
http://dx.doi.org/10.3389/fgene.2020.00203
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