Cargando…
Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease
Studies of inflammatory bowel disease (IBD) have been inconclusive in relating microbiota with distribution of inflammation. We report microbiota, host transcriptomics, epigenomics and genetics from matched inflamed and non-inflamed colonic mucosa [50 Crohn’s disease (CD); 80 ulcerative colitis (UC)...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089947/ https://www.ncbi.nlm.nih.gov/pubmed/32251296 http://dx.doi.org/10.1038/s41467-020-15342-5 |
| _version_ | 1783509826000650240 |
|---|---|
| author | Ryan, F. J. Ahern, A. M. Fitzgerald, R. S. Laserna-Mendieta, E. J. Power, E. M. Clooney, A. G. O’Donoghue, K. W. McMurdie, P. J. Iwai, S. Crits-Christoph, A. Sheehan, D. Moran, C. Flemer, B. Zomer, A. L. Fanning, A. O’Callaghan, J. Walton, J. Temko, A. Stack, W. Jackson, L. Joyce, S. A. Melgar, S. DeSantis, T. Z. Bell, J. T. Shanahan, F. Claesson, M. J. |
| author_facet | Ryan, F. J. Ahern, A. M. Fitzgerald, R. S. Laserna-Mendieta, E. J. Power, E. M. Clooney, A. G. O’Donoghue, K. W. McMurdie, P. J. Iwai, S. Crits-Christoph, A. Sheehan, D. Moran, C. Flemer, B. Zomer, A. L. Fanning, A. O’Callaghan, J. Walton, J. Temko, A. Stack, W. Jackson, L. Joyce, S. A. Melgar, S. DeSantis, T. Z. Bell, J. T. Shanahan, F. Claesson, M. J. |
| author_sort | Ryan, F. J. |
| collection | PubMed |
| description | Studies of inflammatory bowel disease (IBD) have been inconclusive in relating microbiota with distribution of inflammation. We report microbiota, host transcriptomics, epigenomics and genetics from matched inflamed and non-inflamed colonic mucosa [50 Crohn’s disease (CD); 80 ulcerative colitis (UC); 31 controls]. Changes in community-wide and within-patient microbiota are linked with inflammation, but we find no evidence for a distinct microbial diagnostic signature, probably due to heterogeneous host-microbe interactions, and show only marginal microbiota associations with habitual diet. Epithelial DNA methylation improves disease classification and is associated with both inflammation and microbiota composition. Microbiota sub-groups are driven by dominant Enterbacteriaceae and Bacteroides species, representative strains of which are pro-inflammatory in vitro, are also associated with immune-related epigenetic markers. In conclusion, inflamed and non-inflamed colonic segments in both CD and UC differ in microbiota composition and epigenetic profiles. |
| format | Online Article Text |
| id | pubmed-7089947 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-70899472020-03-26 Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease Ryan, F. J. Ahern, A. M. Fitzgerald, R. S. Laserna-Mendieta, E. J. Power, E. M. Clooney, A. G. O’Donoghue, K. W. McMurdie, P. J. Iwai, S. Crits-Christoph, A. Sheehan, D. Moran, C. Flemer, B. Zomer, A. L. Fanning, A. O’Callaghan, J. Walton, J. Temko, A. Stack, W. Jackson, L. Joyce, S. A. Melgar, S. DeSantis, T. Z. Bell, J. T. Shanahan, F. Claesson, M. J. Nat Commun Article Studies of inflammatory bowel disease (IBD) have been inconclusive in relating microbiota with distribution of inflammation. We report microbiota, host transcriptomics, epigenomics and genetics from matched inflamed and non-inflamed colonic mucosa [50 Crohn’s disease (CD); 80 ulcerative colitis (UC); 31 controls]. Changes in community-wide and within-patient microbiota are linked with inflammation, but we find no evidence for a distinct microbial diagnostic signature, probably due to heterogeneous host-microbe interactions, and show only marginal microbiota associations with habitual diet. Epithelial DNA methylation improves disease classification and is associated with both inflammation and microbiota composition. Microbiota sub-groups are driven by dominant Enterbacteriaceae and Bacteroides species, representative strains of which are pro-inflammatory in vitro, are also associated with immune-related epigenetic markers. In conclusion, inflamed and non-inflamed colonic segments in both CD and UC differ in microbiota composition and epigenetic profiles. Nature Publishing Group UK 2020-03-23 /pmc/articles/PMC7089947/ /pubmed/32251296 http://dx.doi.org/10.1038/s41467-020-15342-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Ryan, F. J. Ahern, A. M. Fitzgerald, R. S. Laserna-Mendieta, E. J. Power, E. M. Clooney, A. G. O’Donoghue, K. W. McMurdie, P. J. Iwai, S. Crits-Christoph, A. Sheehan, D. Moran, C. Flemer, B. Zomer, A. L. Fanning, A. O’Callaghan, J. Walton, J. Temko, A. Stack, W. Jackson, L. Joyce, S. A. Melgar, S. DeSantis, T. Z. Bell, J. T. Shanahan, F. Claesson, M. J. Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title | Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title_full | Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title_fullStr | Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title_full_unstemmed | Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title_short | Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| title_sort | colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089947/ https://www.ncbi.nlm.nih.gov/pubmed/32251296 http://dx.doi.org/10.1038/s41467-020-15342-5 |
| work_keys_str_mv | AT ryanfj colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT ahernam colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT fitzgeraldrs colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT lasernamendietaej colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT powerem colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT clooneyag colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT odonoghuekw colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT mcmurdiepj colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT iwais colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT critschristopha colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT sheehand colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT moranc colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT flemerb colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT zomeral colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT fanninga colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT ocallaghanj colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT waltonj colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT temkoa colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT stackw colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT jacksonl colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT joycesa colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT melgars colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT desantistz colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT belljt colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT shanahanf colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease AT claessonmj colonicmicrobiotaisassociatedwithinflammationandhostepigenomicalterationsininflammatoryboweldisease |