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Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women
Specific causes of preterm birth remain unclear. Several recent studies have suggested that immune changes during pregnancy are associated with the timing of delivery, yet few studies have been performed in low-income country settings where the rates of preterm birth are the highest. We conducted a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089959/ https://www.ncbi.nlm.nih.gov/pubmed/32256497 http://dx.doi.org/10.3389/fimmu.2020.00452 |
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author | Seiler, Christof Bayless, Nicholas L. Vergara, Rosemary Pintye, Jillian Kinuthia, John Osborn, Lusi Matemo, Daniel Richardson, Barbra A. John-Stewart, Grace Holmes, Susan Blish, Catherine A. |
author_facet | Seiler, Christof Bayless, Nicholas L. Vergara, Rosemary Pintye, Jillian Kinuthia, John Osborn, Lusi Matemo, Daniel Richardson, Barbra A. John-Stewart, Grace Holmes, Susan Blish, Catherine A. |
author_sort | Seiler, Christof |
collection | PubMed |
description | Specific causes of preterm birth remain unclear. Several recent studies have suggested that immune changes during pregnancy are associated with the timing of delivery, yet few studies have been performed in low-income country settings where the rates of preterm birth are the highest. We conducted a retrospective nested case-control evaluation within a longitudinal study among HIV-uninfected pregnant Kenyan women. To characterize immune function in these women, we evaluated unstimulated and stimulated peripheral blood mononuclear cells in vitro with the A/California/2009 strain of influenza to understand the influenza-induced immune response. We then evaluated transcript expression profiles using the Affymetrix Human GeneChip Transcriptome Array 2.0. Transcriptional profiles of sufficient quality for analysis were obtained from 54 women; 19 of these women delivered <34 weeks and were defined as preterm cases and 35 controls delivered >37 weeks. The median time to birth from sample collection was 13 weeks. No transcripts were significantly associated with preterm birth in a case-control study of matched term and preterm birth (n = 42 women). In the influenza-stimulated samples, expression of IFNL1 was associated with longer time to delivery—the amount of time between sample collection and delivery (n = 54 women). A qPCR analysis confirmed that influenza-induced IFNL expression was associated with longer time to delivery. These data indicate that during pregnancy, ex vivo influenza stimulation results in altered transcriptional response and is associated with time to delivery in cohort of women residing in an area with high preterm birth prevalence. |
format | Online Article Text |
id | pubmed-7089959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70899592020-03-31 Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women Seiler, Christof Bayless, Nicholas L. Vergara, Rosemary Pintye, Jillian Kinuthia, John Osborn, Lusi Matemo, Daniel Richardson, Barbra A. John-Stewart, Grace Holmes, Susan Blish, Catherine A. Front Immunol Immunology Specific causes of preterm birth remain unclear. Several recent studies have suggested that immune changes during pregnancy are associated with the timing of delivery, yet few studies have been performed in low-income country settings where the rates of preterm birth are the highest. We conducted a retrospective nested case-control evaluation within a longitudinal study among HIV-uninfected pregnant Kenyan women. To characterize immune function in these women, we evaluated unstimulated and stimulated peripheral blood mononuclear cells in vitro with the A/California/2009 strain of influenza to understand the influenza-induced immune response. We then evaluated transcript expression profiles using the Affymetrix Human GeneChip Transcriptome Array 2.0. Transcriptional profiles of sufficient quality for analysis were obtained from 54 women; 19 of these women delivered <34 weeks and were defined as preterm cases and 35 controls delivered >37 weeks. The median time to birth from sample collection was 13 weeks. No transcripts were significantly associated with preterm birth in a case-control study of matched term and preterm birth (n = 42 women). In the influenza-stimulated samples, expression of IFNL1 was associated with longer time to delivery—the amount of time between sample collection and delivery (n = 54 women). A qPCR analysis confirmed that influenza-induced IFNL expression was associated with longer time to delivery. These data indicate that during pregnancy, ex vivo influenza stimulation results in altered transcriptional response and is associated with time to delivery in cohort of women residing in an area with high preterm birth prevalence. Frontiers Media S.A. 2020-03-17 /pmc/articles/PMC7089959/ /pubmed/32256497 http://dx.doi.org/10.3389/fimmu.2020.00452 Text en Copyright © 2020 Seiler, Bayless, Vergara, Pintye, Kinuthia, Osborn, Matemo, Richardson, John-Stewart, Holmes and Blish. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Seiler, Christof Bayless, Nicholas L. Vergara, Rosemary Pintye, Jillian Kinuthia, John Osborn, Lusi Matemo, Daniel Richardson, Barbra A. John-Stewart, Grace Holmes, Susan Blish, Catherine A. Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title | Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title_full | Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title_fullStr | Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title_full_unstemmed | Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title_short | Influenza-Induced Interferon Lambda Response Is Associated With Longer Time to Delivery Among Pregnant Kenyan Women |
title_sort | influenza-induced interferon lambda response is associated with longer time to delivery among pregnant kenyan women |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089959/ https://www.ncbi.nlm.nih.gov/pubmed/32256497 http://dx.doi.org/10.3389/fimmu.2020.00452 |
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