Cargando…
Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus
PURPOSE: The purpose of this study was to explore the difference and association between intestinal microbiota and plasma metabolomics between type 2 diabetes mellitus (T2DM) and normal group and to identify potential microbiota biomarkers that contribute the most to the difference in metabolites. M...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090210/ https://www.ncbi.nlm.nih.gov/pubmed/32256098 http://dx.doi.org/10.2147/DMSO.S240728 |
_version_ | 1783509886055743488 |
---|---|
author | Wang, Yuxin Ouyang, Meishuo Gao, Xibao Wang, Shuai Fu, Chunyang Zeng, Jiayi He, Xiaodong |
author_facet | Wang, Yuxin Ouyang, Meishuo Gao, Xibao Wang, Shuai Fu, Chunyang Zeng, Jiayi He, Xiaodong |
author_sort | Wang, Yuxin |
collection | PubMed |
description | PURPOSE: The purpose of this study was to explore the difference and association between intestinal microbiota and plasma metabolomics between type 2 diabetes mellitus (T2DM) and normal group and to identify potential microbiota biomarkers that contribute the most to the difference in metabolites. METHODS: Six male ZDF model (fa/fa) rats were fed by a Purina #5008 Lab Diet (crude protein 23.5%, crude fat 6.5%) for 3 weeks and their age-matched 6 ZDF control (fa/+) rats were fed by normal rodent diet. Their stool and blood samples were collected at 12 weeks. To analyze the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Liquid chromatography–mass spectrometry (LC-MS) followed by multivariate statistical analysis was applied to the plasma metabolites profiling. Correlation analysis of them was calculated by Pearson statistical method. RESULTS: Twelve potential biomarkers of intestinal microbial flora and 357 differential metabolites were found in ZDF fa/fa rats, among which there are three flora that contributed the most to the perturbation of metabolites, including genus Phocea, Pseudoflavonifractor and species Lactobacillus intestinalis. CONCLUSION: Our study demonstrates the alterations of the abundance and diversity of the intestinal microbiota and the perturbation of metabolites in ZDF rats (fa/fa). We found three potential biomarkers of intestinal microbiota that may lead to perturbation in plasma metabolites. This may prompt new pathogenesis of obesity-related T2DM, but we also need to study further about the causal relationship between intestinal microbe and T2DM, so as to find the target of T2DM treatment or preventive measures. |
format | Online Article Text |
id | pubmed-7090210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-70902102020-04-01 Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus Wang, Yuxin Ouyang, Meishuo Gao, Xibao Wang, Shuai Fu, Chunyang Zeng, Jiayi He, Xiaodong Diabetes Metab Syndr Obes Original Research PURPOSE: The purpose of this study was to explore the difference and association between intestinal microbiota and plasma metabolomics between type 2 diabetes mellitus (T2DM) and normal group and to identify potential microbiota biomarkers that contribute the most to the difference in metabolites. METHODS: Six male ZDF model (fa/fa) rats were fed by a Purina #5008 Lab Diet (crude protein 23.5%, crude fat 6.5%) for 3 weeks and their age-matched 6 ZDF control (fa/+) rats were fed by normal rodent diet. Their stool and blood samples were collected at 12 weeks. To analyze the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Liquid chromatography–mass spectrometry (LC-MS) followed by multivariate statistical analysis was applied to the plasma metabolites profiling. Correlation analysis of them was calculated by Pearson statistical method. RESULTS: Twelve potential biomarkers of intestinal microbial flora and 357 differential metabolites were found in ZDF fa/fa rats, among which there are three flora that contributed the most to the perturbation of metabolites, including genus Phocea, Pseudoflavonifractor and species Lactobacillus intestinalis. CONCLUSION: Our study demonstrates the alterations of the abundance and diversity of the intestinal microbiota and the perturbation of metabolites in ZDF rats (fa/fa). We found three potential biomarkers of intestinal microbiota that may lead to perturbation in plasma metabolites. This may prompt new pathogenesis of obesity-related T2DM, but we also need to study further about the causal relationship between intestinal microbe and T2DM, so as to find the target of T2DM treatment or preventive measures. Dove 2020-03-19 /pmc/articles/PMC7090210/ /pubmed/32256098 http://dx.doi.org/10.2147/DMSO.S240728 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Wang, Yuxin Ouyang, Meishuo Gao, Xibao Wang, Shuai Fu, Chunyang Zeng, Jiayi He, Xiaodong Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title | Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title_full | Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title_fullStr | Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title_full_unstemmed | Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title_short | Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus |
title_sort | phocea, pseudoflavonifractor and lactobacillus intestinalis: three potential biomarkers of gut microbiota that affect progression and complications of obesity-induced type 2 diabetes mellitus |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090210/ https://www.ncbi.nlm.nih.gov/pubmed/32256098 http://dx.doi.org/10.2147/DMSO.S240728 |
work_keys_str_mv | AT wangyuxin phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT ouyangmeishuo phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT gaoxibao phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT wangshuai phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT fuchunyang phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT zengjiayi phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus AT hexiaodong phoceapseudoflavonifractorandlactobacillusintestinalisthreepotentialbiomarkersofgutmicrobiotathataffectprogressionandcomplicationsofobesityinducedtype2diabetesmellitus |