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High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth

Haematococcus lacustris (H. lacustris), a promising source for astaxanthin production, is a light-sensitive microalga that is prone to sluggish growth when subjected to high levels of irradiance. A challenge in H. lacustris culture is to find a way to efficiently use illumination to maintain vigorou...

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Detalles Bibliográficos
Autores principales: Wu, Kebi, Ying, Kezhen, Liu, Lu, Zhou, Jin, Cai, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090360/
https://www.ncbi.nlm.nih.gov/pubmed/32215258
http://dx.doi.org/10.1016/j.btre.2020.e00444
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author Wu, Kebi
Ying, Kezhen
Liu, Lu
Zhou, Jin
Cai, Zhonghua
author_facet Wu, Kebi
Ying, Kezhen
Liu, Lu
Zhou, Jin
Cai, Zhonghua
author_sort Wu, Kebi
collection PubMed
description Haematococcus lacustris (H. lacustris), a promising source for astaxanthin production, is a light-sensitive microalga that is prone to sluggish growth when subjected to high levels of irradiance. A challenge in H. lacustris culture is to find a way to efficiently use illumination to maintain vigorous growth and harvest dense biomass, which is essential for further exploiting the potential for astaxanthin production. Previous studies have shown that in addition to illumination, carbon supply in culture is a key limitation for algae growth. Here, we investigated a combined culture approach involving high light intensity (110 μmol m(−2)s(-1)) and injection of a 1% (v/v) CO(2) air-gas mixture which provided an effective method for H. lacustris culture to achieve both a high growth rate and high cell density. The cell number in the group with high light exposure combined with CO(2) enrichment was increased almost four-fold compared with a high light group (110 μmol m(−2)s(-1) without CO(2) injection). Additional experiments suggested a possible mechanism in which elevated CO(2) increases the electron sink capacity, thus alleviating photoinhibition and oxidative damage. The scaled-up photobioreactor demonstrated much better performance, with growth rates improved by 50–350 %, providing further evidence that this new method can improve algal cell production. Overall, our work provides an efficient way for H. lacustris culture and manufacture, with potential industrial applications.
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spelling pubmed-70903602020-03-25 High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth Wu, Kebi Ying, Kezhen Liu, Lu Zhou, Jin Cai, Zhonghua Biotechnol Rep (Amst) Articles from the Special Issue on Microbial technology for the development of sustainable energy and environment; Edited by Xiaobo Liu Haematococcus lacustris (H. lacustris), a promising source for astaxanthin production, is a light-sensitive microalga that is prone to sluggish growth when subjected to high levels of irradiance. A challenge in H. lacustris culture is to find a way to efficiently use illumination to maintain vigorous growth and harvest dense biomass, which is essential for further exploiting the potential for astaxanthin production. Previous studies have shown that in addition to illumination, carbon supply in culture is a key limitation for algae growth. Here, we investigated a combined culture approach involving high light intensity (110 μmol m(−2)s(-1)) and injection of a 1% (v/v) CO(2) air-gas mixture which provided an effective method for H. lacustris culture to achieve both a high growth rate and high cell density. The cell number in the group with high light exposure combined with CO(2) enrichment was increased almost four-fold compared with a high light group (110 μmol m(−2)s(-1) without CO(2) injection). Additional experiments suggested a possible mechanism in which elevated CO(2) increases the electron sink capacity, thus alleviating photoinhibition and oxidative damage. The scaled-up photobioreactor demonstrated much better performance, with growth rates improved by 50–350 %, providing further evidence that this new method can improve algal cell production. Overall, our work provides an efficient way for H. lacustris culture and manufacture, with potential industrial applications. Elsevier 2020-03-12 /pmc/articles/PMC7090360/ /pubmed/32215258 http://dx.doi.org/10.1016/j.btre.2020.e00444 Text en © 2020 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles from the Special Issue on Microbial technology for the development of sustainable energy and environment; Edited by Xiaobo Liu
Wu, Kebi
Ying, Kezhen
Liu, Lu
Zhou, Jin
Cai, Zhonghua
High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title_full High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title_fullStr High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title_full_unstemmed High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title_short High irradiance compensated with CO(2) enhances the efficiency of Haematococcus lacustris growth
title_sort high irradiance compensated with co(2) enhances the efficiency of haematococcus lacustris growth
topic Articles from the Special Issue on Microbial technology for the development of sustainable energy and environment; Edited by Xiaobo Liu
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090360/
https://www.ncbi.nlm.nih.gov/pubmed/32215258
http://dx.doi.org/10.1016/j.btre.2020.e00444
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