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Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture

A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells w...

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Autores principales: Xiang, Yang-fei, Ju, Huai-qiang, Li, Shen, Zhang, Ying-jun, Yang, Chong-ren, Wang, Yi-fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Wuhan Institute of Virology, CAS 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090425/
https://www.ncbi.nlm.nih.gov/pubmed/20960184
http://dx.doi.org/10.1007/s12250-010-3144-y
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author Xiang, Yang-fei
Ju, Huai-qiang
Li, Shen
Zhang, Ying-jun
Yang, Chong-ren
Wang, Yi-fei
author_facet Xiang, Yang-fei
Ju, Huai-qiang
Li, Shen
Zhang, Ying-jun
Yang, Chong-ren
Wang, Yi-fei
author_sort Xiang, Yang-fei
collection PubMed
description A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.
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spelling pubmed-70904252020-03-24 Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture Xiang, Yang-fei Ju, Huai-qiang Li, Shen Zhang, Ying-jun Yang, Chong-ren Wang, Yi-fei Virol Sin Article A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG. SP Wuhan Institute of Virology, CAS 2010-10-08 /pmc/articles/PMC7090425/ /pubmed/20960184 http://dx.doi.org/10.1007/s12250-010-3144-y Text en © Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010
spellingShingle Article
Xiang, Yang-fei
Ju, Huai-qiang
Li, Shen
Zhang, Ying-jun
Yang, Chong-ren
Wang, Yi-fei
Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title_full Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title_fullStr Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title_full_unstemmed Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title_short Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
title_sort effects of 1,2,4,6-tetra-o-galloyl-β-d-glucose from p. emblica on hbsag and hbeag secretion in hepg2.2.15 cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090425/
https://www.ncbi.nlm.nih.gov/pubmed/20960184
http://dx.doi.org/10.1007/s12250-010-3144-y
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