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Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture
A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SP Wuhan Institute of Virology, CAS
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090425/ https://www.ncbi.nlm.nih.gov/pubmed/20960184 http://dx.doi.org/10.1007/s12250-010-3144-y |
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author | Xiang, Yang-fei Ju, Huai-qiang Li, Shen Zhang, Ying-jun Yang, Chong-ren Wang, Yi-fei |
author_facet | Xiang, Yang-fei Ju, Huai-qiang Li, Shen Zhang, Ying-jun Yang, Chong-ren Wang, Yi-fei |
author_sort | Xiang, Yang-fei |
collection | PubMed |
description | A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG. |
format | Online Article Text |
id | pubmed-7090425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | SP Wuhan Institute of Virology, CAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-70904252020-03-24 Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture Xiang, Yang-fei Ju, Huai-qiang Li, Shen Zhang, Ying-jun Yang, Chong-ren Wang, Yi-fei Virol Sin Article A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG. SP Wuhan Institute of Virology, CAS 2010-10-08 /pmc/articles/PMC7090425/ /pubmed/20960184 http://dx.doi.org/10.1007/s12250-010-3144-y Text en © Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010 |
spellingShingle | Article Xiang, Yang-fei Ju, Huai-qiang Li, Shen Zhang, Ying-jun Yang, Chong-ren Wang, Yi-fei Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title | Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title_full | Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title_fullStr | Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title_full_unstemmed | Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title_short | Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture |
title_sort | effects of 1,2,4,6-tetra-o-galloyl-β-d-glucose from p. emblica on hbsag and hbeag secretion in hepg2.2.15 cell culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090425/ https://www.ncbi.nlm.nih.gov/pubmed/20960184 http://dx.doi.org/10.1007/s12250-010-3144-y |
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