Cxcl16 interact with SARS-CoV N protein in and out cell

Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The H...

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Detalles Bibliográficos
Autores principales: Zhang, Yuan-peng, Zhang, Rong-wu, Chang, Wei-shan, Wang, Yan-yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Wuhan Institute of Virology, CAS 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090476/
https://www.ncbi.nlm.nih.gov/pubmed/20960183
http://dx.doi.org/10.1007/s12250-010-3129-x
Descripción
Sumario:Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The HIS-tagged fusion protein SARS-CoVN-GFP was then built and purified for the binding assay in vitro. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. It is suggested that their interaction might be through direct combination. Under a fluorescence microscope, it was observed that the purified fusion protein SARS-CoVN-GFP was attached to the cell membrane of CXCL16-transfected cells, indicating that SARS-CoVN and CXCL16 can be mutually combined.

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