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An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells

In this study we examine the possibility that TiO(2) nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO(2) nanoparticles, which were obtained as colloidal solutions at a...

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Autores principales: Zarytova, V. F., Zinov’ev, V. V., Ismagilov, Z. R., Levina, A. S., Repkova, M. N., Shikina, N. V., Evdokimov, A. A., Belanov, E. F., Balakhnin, S. M., Serova, O. A., Baiborodin, S. I., Malygin, E. G., Zagrebel’nyi, S. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP MAIK Nauka/Interperiodica 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090768/
https://www.ncbi.nlm.nih.gov/pubmed/32218897
http://dx.doi.org/10.1134/S1995078009090158
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author Zarytova, V. F.
Zinov’ev, V. V.
Ismagilov, Z. R.
Levina, A. S.
Repkova, M. N.
Shikina, N. V.
Evdokimov, A. A.
Belanov, E. F.
Balakhnin, S. M.
Serova, O. A.
Baiborodin, S. I.
Malygin, E. G.
Zagrebel’nyi, S. N.
author_facet Zarytova, V. F.
Zinov’ev, V. V.
Ismagilov, Z. R.
Levina, A. S.
Repkova, M. N.
Shikina, N. V.
Evdokimov, A. A.
Belanov, E. F.
Balakhnin, S. M.
Serova, O. A.
Baiborodin, S. I.
Malygin, E. G.
Zagrebel’nyi, S. N.
author_sort Zarytova, V. F.
collection PubMed
description In this study we examine the possibility that TiO(2) nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO(2) nanoparticles, which were obtained as colloidal solutions at a concentration of TiO(2) 0.3M by TiCl(4) hydrolysis. The electronic microscopy of various cell cultures (KCT, Vero, and MDCK) treated with nanoparticle solutions (20 µg/µl) showed that nanoparticles could enter the cells and accumulate in the vacuoles and phagosomes and form inclusions in cytoplasm. Thus, we demonstrated the penetration of TiO(2) nanoparticles and their oligonucleotide conjugates into intracellular space without any auxiliary operations. Most other researches used electroporation techniques for similar purposes [1, 2, 5].
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spelling pubmed-70907682020-03-24 An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells Zarytova, V. F. Zinov’ev, V. V. Ismagilov, Z. R. Levina, A. S. Repkova, M. N. Shikina, N. V. Evdokimov, A. A. Belanov, E. F. Balakhnin, S. M. Serova, O. A. Baiborodin, S. I. Malygin, E. G. Zagrebel’nyi, S. N. Nanotechnol Russ Articles In this study we examine the possibility that TiO(2) nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO(2) nanoparticles, which were obtained as colloidal solutions at a concentration of TiO(2) 0.3M by TiCl(4) hydrolysis. The electronic microscopy of various cell cultures (KCT, Vero, and MDCK) treated with nanoparticle solutions (20 µg/µl) showed that nanoparticles could enter the cells and accumulate in the vacuoles and phagosomes and form inclusions in cytoplasm. Thus, we demonstrated the penetration of TiO(2) nanoparticles and their oligonucleotide conjugates into intracellular space without any auxiliary operations. Most other researches used electroporation techniques for similar purposes [1, 2, 5]. SP MAIK Nauka/Interperiodica 2009-10-20 2009 /pmc/articles/PMC7090768/ /pubmed/32218897 http://dx.doi.org/10.1134/S1995078009090158 Text en © Pleiades Publishing, Ltd. 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Articles
Zarytova, V. F.
Zinov’ev, V. V.
Ismagilov, Z. R.
Levina, A. S.
Repkova, M. N.
Shikina, N. V.
Evdokimov, A. A.
Belanov, E. F.
Balakhnin, S. M.
Serova, O. A.
Baiborodin, S. I.
Malygin, E. G.
Zagrebel’nyi, S. N.
An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title_full An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title_fullStr An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title_full_unstemmed An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title_short An examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
title_sort examination of the ability of titanium dioxide nanoparticles and its conjugates with oligonucleotides to penetrate into eucariotis cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090768/
https://www.ncbi.nlm.nih.gov/pubmed/32218897
http://dx.doi.org/10.1134/S1995078009090158
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