Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO(4)) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO(4) strong...

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Detalles Bibliográficos
Autores principales: Fani, Mona, Khodadad, Nastaran, Ebrahimi, Saeedeh, Nahidsamiei, Rahil, Makvandi, Manoocher, Teimoori, Ali, Langari, Hadis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090960/
https://www.ncbi.nlm.nih.gov/pubmed/31028520
http://dx.doi.org/10.1007/s12011-019-01728-0
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author Fani, Mona
Khodadad, Nastaran
Ebrahimi, Saeedeh
Nahidsamiei, Rahil
Makvandi, Manoocher
Teimoori, Ali
Langari, Hadis
author_facet Fani, Mona
Khodadad, Nastaran
Ebrahimi, Saeedeh
Nahidsamiei, Rahil
Makvandi, Manoocher
Teimoori, Ali
Langari, Hadis
author_sort Fani, Mona
collection PubMed
description This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO(4)) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO(4) strongly inhibited the replication of virus progeny (MOI 0.001) at least 68-fold less. On the other hand, the IC50 demonstrated that the highest activity of ZnSO(4) was at the 0.23 mM concentration.
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spelling pubmed-70909602020-03-24 Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay Fani, Mona Khodadad, Nastaran Ebrahimi, Saeedeh Nahidsamiei, Rahil Makvandi, Manoocher Teimoori, Ali Langari, Hadis Biol Trace Elem Res Article This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO(4)) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO(4) strongly inhibited the replication of virus progeny (MOI 0.001) at least 68-fold less. On the other hand, the IC50 demonstrated that the highest activity of ZnSO(4) was at the 0.23 mM concentration. Springer US 2019-04-26 2020 /pmc/articles/PMC7090960/ /pubmed/31028520 http://dx.doi.org/10.1007/s12011-019-01728-0 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2019 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Fani, Mona
Khodadad, Nastaran
Ebrahimi, Saeedeh
Nahidsamiei, Rahil
Makvandi, Manoocher
Teimoori, Ali
Langari, Hadis
Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title_full Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title_fullStr Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title_full_unstemmed Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title_short Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay
title_sort zinc sulfate in narrow range as an in vitro anti-hsv-1 assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090960/
https://www.ncbi.nlm.nih.gov/pubmed/31028520
http://dx.doi.org/10.1007/s12011-019-01728-0
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