Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain

The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 10(...

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Autores principales: Lee, Jung-Ah, Lee, Nak-Hyung, Lee, Sang-Won, Park, Seung-Yong, Song, Chang-Seon, Choi, In-Soo, Lee, Joong-Bok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Microbiological Society of Korea 2014
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091204/
https://www.ncbi.nlm.nih.gov/pubmed/24682997
http://dx.doi.org/10.1007/s12275-014-4074-4
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author Lee, Jung-Ah
Lee, Nak-Hyung
Lee, Sang-Won
Park, Seung-Yong
Song, Chang-Seon
Choi, In-Soo
Lee, Joong-Bok
author_facet Lee, Jung-Ah
Lee, Nak-Hyung
Lee, Sang-Won
Park, Seung-Yong
Song, Chang-Seon
Choi, In-Soo
Lee, Joong-Bok
author_sort Lee, Jung-Ah
collection PubMed
description The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 10(6) TCID(50)/ml of viral titer with similar growth kinetics to those of parental strains and had a cross-reactive neutralizing antibody response to field serum from the entire country. The chimeric clone pK418 can be used as a practical tool for further studying the molecular characteristics of PRRSV proteins through genetic manipulation. Furthermore, successful construction of the K418 will allow for the development of customized vaccine candidates against PRRSV, which has evolved rapidly in Korea.
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spelling pubmed-70912042020-03-24 Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain Lee, Jung-Ah Lee, Nak-Hyung Lee, Sang-Won Park, Seung-Yong Song, Chang-Seon Choi, In-Soo Lee, Joong-Bok J Microbiol Virology The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 10(6) TCID(50)/ml of viral titer with similar growth kinetics to those of parental strains and had a cross-reactive neutralizing antibody response to field serum from the entire country. The chimeric clone pK418 can be used as a practical tool for further studying the molecular characteristics of PRRSV proteins through genetic manipulation. Furthermore, successful construction of the K418 will allow for the development of customized vaccine candidates against PRRSV, which has evolved rapidly in Korea. The Microbiological Society of Korea 2014-03-29 2014 /pmc/articles/PMC7091204/ /pubmed/24682997 http://dx.doi.org/10.1007/s12275-014-4074-4 Text en © The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Virology
Lee, Jung-Ah
Lee, Nak-Hyung
Lee, Sang-Won
Park, Seung-Yong
Song, Chang-Seon
Choi, In-Soo
Lee, Joong-Bok
Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title_full Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title_fullStr Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title_full_unstemmed Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title_short Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain
title_sort development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a korean dominant field strain
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091204/
https://www.ncbi.nlm.nih.gov/pubmed/24682997
http://dx.doi.org/10.1007/s12275-014-4074-4
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