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CSFV proliferation is associated with GBF1 and Rab2

The Golgi apparatus and its resident proteins are utilized and regulated by viruses to facilitate their proliferation. In this study, we investigated Classical swine fever virus (CSFV) proliferation when the function of the Golgi was disturbed. Golgi function was disturbed using chemical inhibitors,...

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Detalles Bibliográficos
Autores principales: Liang, Wulong, Zheng, Minping, Bao, Changlei, Zhang, Yanming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer India 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091326/
https://www.ncbi.nlm.nih.gov/pubmed/28229964
http://dx.doi.org/10.1007/s12038-016-9659-0
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author Liang, Wulong
Zheng, Minping
Bao, Changlei
Zhang, Yanming
author_facet Liang, Wulong
Zheng, Minping
Bao, Changlei
Zhang, Yanming
author_sort Liang, Wulong
collection PubMed
description The Golgi apparatus and its resident proteins are utilized and regulated by viruses to facilitate their proliferation. In this study, we investigated Classical swine fever virus (CSFV) proliferation when the function of the Golgi was disturbed. Golgi function was disturbed using chemical inhibitors, namely, brefeldin A (BFA) and golgicide A (GCA), and RNA interfering targets, such as the Golgi-specific BFA-resistance guanine nucleotide exchange factor 1 (GBF1) and Rab2 GTPases. CSFV proliferation was significantly inhibited during RNA replication and viral particle generation after BFA and GCA treatment. CSFV multiplication dynamics were retarded in cells transfected with GBF1 and Rab2 shRNA. Furthermore, CSFV proliferation was promoted by GBF1 and Rab2 overexpression using a lentiviral system. Hence, Golgi function is important for CSFV multiplication, and GBF1 and Rab2 participate in CSFV proliferation. Further studies must investigate Golgi-resident proteins to elucidate the mechanism underlying CSFV replication.
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spelling pubmed-70913262020-03-24 CSFV proliferation is associated with GBF1 and Rab2 Liang, Wulong Zheng, Minping Bao, Changlei Zhang, Yanming J Biosci Article The Golgi apparatus and its resident proteins are utilized and regulated by viruses to facilitate their proliferation. In this study, we investigated Classical swine fever virus (CSFV) proliferation when the function of the Golgi was disturbed. Golgi function was disturbed using chemical inhibitors, namely, brefeldin A (BFA) and golgicide A (GCA), and RNA interfering targets, such as the Golgi-specific BFA-resistance guanine nucleotide exchange factor 1 (GBF1) and Rab2 GTPases. CSFV proliferation was significantly inhibited during RNA replication and viral particle generation after BFA and GCA treatment. CSFV multiplication dynamics were retarded in cells transfected with GBF1 and Rab2 shRNA. Furthermore, CSFV proliferation was promoted by GBF1 and Rab2 overexpression using a lentiviral system. Hence, Golgi function is important for CSFV multiplication, and GBF1 and Rab2 participate in CSFV proliferation. Further studies must investigate Golgi-resident proteins to elucidate the mechanism underlying CSFV replication. Springer India 2016-12-29 2017 /pmc/articles/PMC7091326/ /pubmed/28229964 http://dx.doi.org/10.1007/s12038-016-9659-0 Text en © Indian Academy of Sciences 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Liang, Wulong
Zheng, Minping
Bao, Changlei
Zhang, Yanming
CSFV proliferation is associated with GBF1 and Rab2
title CSFV proliferation is associated with GBF1 and Rab2
title_full CSFV proliferation is associated with GBF1 and Rab2
title_fullStr CSFV proliferation is associated with GBF1 and Rab2
title_full_unstemmed CSFV proliferation is associated with GBF1 and Rab2
title_short CSFV proliferation is associated with GBF1 and Rab2
title_sort csfv proliferation is associated with gbf1 and rab2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091326/
https://www.ncbi.nlm.nih.gov/pubmed/28229964
http://dx.doi.org/10.1007/s12038-016-9659-0
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