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Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the se...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091820/ https://www.ncbi.nlm.nih.gov/pubmed/15780182 http://dx.doi.org/10.1038/sj.cr.7290286 |
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author | SHI, Yi YANG, De Hua XIONG, Jie JIA, Jie HUANG, Bing JIN, You Xin |
author_facet | SHI, Yi YANG, De Hua XIONG, Jie JIA, Jie HUANG, Bing JIN, You Xin |
author_sort | SHI, Yi |
collection | PubMed |
description | RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3′ overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0∼60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS. |
format | Online Article Text |
id | pubmed-7091820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70918202020-03-24 Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs SHI, Yi YANG, De Hua XIONG, Jie JIA, Jie HUANG, Bing JIN, You Xin Cell Res Article RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3′ overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0∼60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS. Nature Publishing Group UK 2005-03 /pmc/articles/PMC7091820/ /pubmed/15780182 http://dx.doi.org/10.1038/sj.cr.7290286 Text en © Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article SHI, Yi YANG, De Hua XIONG, Jie JIA, Jie HUANG, Bing JIN, You Xin Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title | Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title_full | Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title_fullStr | Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title_full_unstemmed | Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title_short | Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs |
title_sort | inhibition of genes expression of sars coronavirus by synthetic small interfering rnas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091820/ https://www.ncbi.nlm.nih.gov/pubmed/15780182 http://dx.doi.org/10.1038/sj.cr.7290286 |
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