Cargando…

Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs

RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the se...

Descripción completa

Detalles Bibliográficos
Autores principales: SHI, Yi, YANG, De Hua, XIONG, Jie, JIA, Jie, HUANG, Bing, JIN, You Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091820/
https://www.ncbi.nlm.nih.gov/pubmed/15780182
http://dx.doi.org/10.1038/sj.cr.7290286
_version_ 1783510066363629568
author SHI, Yi
YANG, De Hua
XIONG, Jie
JIA, Jie
HUANG, Bing
JIN, You Xin
author_facet SHI, Yi
YANG, De Hua
XIONG, Jie
JIA, Jie
HUANG, Bing
JIN, You Xin
author_sort SHI, Yi
collection PubMed
description RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3′ overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0∼60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.
format Online
Article
Text
id pubmed-7091820
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-70918202020-03-24 Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs SHI, Yi YANG, De Hua XIONG, Jie JIA, Jie HUANG, Bing JIN, You Xin Cell Res Article RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3′ overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0∼60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS. Nature Publishing Group UK 2005-03 /pmc/articles/PMC7091820/ /pubmed/15780182 http://dx.doi.org/10.1038/sj.cr.7290286 Text en © Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
SHI, Yi
YANG, De Hua
XIONG, Jie
JIA, Jie
HUANG, Bing
JIN, You Xin
Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title_full Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title_fullStr Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title_full_unstemmed Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title_short Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
title_sort inhibition of genes expression of sars coronavirus by synthetic small interfering rnas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091820/
https://www.ncbi.nlm.nih.gov/pubmed/15780182
http://dx.doi.org/10.1038/sj.cr.7290286
work_keys_str_mv AT shiyi inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas
AT yangdehua inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas
AT xiongjie inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas
AT jiajie inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas
AT huangbing inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas
AT jinyouxin inhibitionofgenesexpressionofsarscoronavirusbysyntheticsmallinterferingrnas