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Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI

BACKGROUND: Peroxiredoxin activated in M-CSF stimulated monocytes (PAMM) is a novel protein produced by adipocytes with putative redox regulatory and anti-inflammatory properties. Because acute spinal cord injury (SCI) is associated with oxidative stress and neuroinflammation and because PAMM can be...

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Autores principales: Morse, Leslie R., Nguyen, Nguyen, Xu, Yan, Jha, Prakash, Battaglino, Ricardo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092454/
https://www.ncbi.nlm.nih.gov/pubmed/32204712
http://dx.doi.org/10.1186/s12967-020-02304-0
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author Morse, Leslie R.
Nguyen, Nguyen
Xu, Yan
Jha, Prakash
Battaglino, Ricardo A.
author_facet Morse, Leslie R.
Nguyen, Nguyen
Xu, Yan
Jha, Prakash
Battaglino, Ricardo A.
author_sort Morse, Leslie R.
collection PubMed
description BACKGROUND: Peroxiredoxin activated in M-CSF stimulated monocytes (PAMM) is a novel protein produced by adipocytes with putative redox regulatory and anti-inflammatory properties. Because acute spinal cord injury (SCI) is associated with oxidative stress and neuroinflammation and because PAMM can be detected in systemic circulation, we hypothesized that acute neuro-trauma might induce changes in circulating PAMM expression. Specifically, we hypothesized that PAMM levels might vary based on the presence or absence of acute, traumatic SCI. We therefore investigated circulating PAMM levels in adults with and without acute traumatic SCI. METHODS: We studied 105 men and women (54 with SCI and 51 without SCI). Participants with SCI were admitted for acute rehabilitation within 1 month after injury. Serum samples were obtained during hospitalization and stored at − 80 °C until batch analysis. Total PAMM was quantified by ELISA assay (MyBiosource, Cat. No: MBS9327247) with a detection limit of 0.25 ng/ml. Separate multivariate models including age, BMI, and injury severity were assessed to determine significant clinical predictors of change in PAMM levels. RESULTS: When adjusting for BMI, age, and gender, mean change in PAMM levels were greatest in participants with motor complete SCI compared to able-bodied (1.65 ng/ml versus 0.94 ng/ml, p = 0.003). This model explained 26% of the variation in change in circulating PAMM levels. CONCLUSIONS: Our results suggest that PAMM may be a novel biomarker of neurological injury or of native anti-inflammatory responses to neurological injury. More work is needed to establish the role of PAMM and other adipocyte-derived factors in the acute response to neurotrauma.
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spelling pubmed-70924542020-03-24 Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI Morse, Leslie R. Nguyen, Nguyen Xu, Yan Jha, Prakash Battaglino, Ricardo A. J Transl Med Research BACKGROUND: Peroxiredoxin activated in M-CSF stimulated monocytes (PAMM) is a novel protein produced by adipocytes with putative redox regulatory and anti-inflammatory properties. Because acute spinal cord injury (SCI) is associated with oxidative stress and neuroinflammation and because PAMM can be detected in systemic circulation, we hypothesized that acute neuro-trauma might induce changes in circulating PAMM expression. Specifically, we hypothesized that PAMM levels might vary based on the presence or absence of acute, traumatic SCI. We therefore investigated circulating PAMM levels in adults with and without acute traumatic SCI. METHODS: We studied 105 men and women (54 with SCI and 51 without SCI). Participants with SCI were admitted for acute rehabilitation within 1 month after injury. Serum samples were obtained during hospitalization and stored at − 80 °C until batch analysis. Total PAMM was quantified by ELISA assay (MyBiosource, Cat. No: MBS9327247) with a detection limit of 0.25 ng/ml. Separate multivariate models including age, BMI, and injury severity were assessed to determine significant clinical predictors of change in PAMM levels. RESULTS: When adjusting for BMI, age, and gender, mean change in PAMM levels were greatest in participants with motor complete SCI compared to able-bodied (1.65 ng/ml versus 0.94 ng/ml, p = 0.003). This model explained 26% of the variation in change in circulating PAMM levels. CONCLUSIONS: Our results suggest that PAMM may be a novel biomarker of neurological injury or of native anti-inflammatory responses to neurological injury. More work is needed to establish the role of PAMM and other adipocyte-derived factors in the acute response to neurotrauma. BioMed Central 2020-03-24 /pmc/articles/PMC7092454/ /pubmed/32204712 http://dx.doi.org/10.1186/s12967-020-02304-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Morse, Leslie R.
Nguyen, Nguyen
Xu, Yan
Jha, Prakash
Battaglino, Ricardo A.
Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title_full Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title_fullStr Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title_full_unstemmed Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title_short Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI
title_sort circulating pamm, a novel antioxidant and anti-inflammatory protein, is elevated in acute sci
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092454/
https://www.ncbi.nlm.nih.gov/pubmed/32204712
http://dx.doi.org/10.1186/s12967-020-02304-0
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