The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate w...

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Autores principales: Zeng, Yingchun, Ye, Linbai, Zhu, Shengli, Zheng, Hong, Zhao, Peng, Cai, Weijia, Su, Liya, She, Yinglong, Wu, Zhenghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092856/
https://www.ncbi.nlm.nih.gov/pubmed/18243139
http://dx.doi.org/10.1016/j.bbrc.2008.01.096
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author Zeng, Yingchun
Ye, Linbai
Zhu, Shengli
Zheng, Hong
Zhao, Peng
Cai, Weijia
Su, Liya
She, Yinglong
Wu, Zhenghui
author_facet Zeng, Yingchun
Ye, Linbai
Zhu, Shengli
Zheng, Hong
Zhao, Peng
Cai, Weijia
Su, Liya
She, Yinglong
Wu, Zhenghui
author_sort Zeng, Yingchun
collection PubMed
description Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate with B23, a phosphoprotein in nucleolus, in vitro and in vivo. Mapping studies localized the critical N sequences for this interaction to amino acid residues 175–210, which included a serine/arginine (SR)-rich domain. In vitro phosphorylation assay showed that the N protein inhibited the B23 phosphorylation at Thr199.
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spelling pubmed-70928562020-03-25 The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation Zeng, Yingchun Ye, Linbai Zhu, Shengli Zheng, Hong Zhao, Peng Cai, Weijia Su, Liya She, Yinglong Wu, Zhenghui Biochem Biophys Res Commun Article Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate with B23, a phosphoprotein in nucleolus, in vitro and in vivo. Mapping studies localized the critical N sequences for this interaction to amino acid residues 175–210, which included a serine/arginine (SR)-rich domain. In vitro phosphorylation assay showed that the N protein inhibited the B23 phosphorylation at Thr199. Published by Elsevier Inc. 2008-05-02 2008-02-01 /pmc/articles/PMC7092856/ /pubmed/18243139 http://dx.doi.org/10.1016/j.bbrc.2008.01.096 Text en Copyright © 2008 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zeng, Yingchun
Ye, Linbai
Zhu, Shengli
Zheng, Hong
Zhao, Peng
Cai, Weijia
Su, Liya
She, Yinglong
Wu, Zhenghui
The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title_full The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title_fullStr The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title_full_unstemmed The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title_short The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
title_sort nucleocapsid protein of sars-associated coronavirus inhibits b23 phosphorylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092856/
https://www.ncbi.nlm.nih.gov/pubmed/18243139
http://dx.doi.org/10.1016/j.bbrc.2008.01.096
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