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Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins
The severe acute respiratory syndrome coronavirus (SARS-CoV) still carries the potential for reemergence, therefore efforts are being made to create a vaccine as a prophylactic strategy for control and prevention. Antibody-dependent enhancement (ADE) is a mechanism through which dengue viruses, feli...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092860/ https://www.ncbi.nlm.nih.gov/pubmed/25073113 http://dx.doi.org/10.1016/j.bbrc.2014.07.090 |
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author | Wang, Sheng-Fan Tseng, Sung-Pin Yen, Chia-Hung Yang, Jyh-Yuan Tsao, Ching-Han Shen, Chun-Wei Chen, Kuan-Hsuan Liu, Fu-Tong Liu, Wu-Tse Chen, Yi-Ming Arthur Huang, Jason C. |
author_facet | Wang, Sheng-Fan Tseng, Sung-Pin Yen, Chia-Hung Yang, Jyh-Yuan Tsao, Ching-Han Shen, Chun-Wei Chen, Kuan-Hsuan Liu, Fu-Tong Liu, Wu-Tse Chen, Yi-Ming Arthur Huang, Jason C. |
author_sort | Wang, Sheng-Fan |
collection | PubMed |
description | The severe acute respiratory syndrome coronavirus (SARS-CoV) still carries the potential for reemergence, therefore efforts are being made to create a vaccine as a prophylactic strategy for control and prevention. Antibody-dependent enhancement (ADE) is a mechanism through which dengue viruses, feline coronaviruses, and HIV viruses take advantage of anti-viral humoral immune responses to infect host target cells. Here we describe our observations of SARS-CoV using ADE to enhance the infectivity of a HL-CZ human promonocyte cell line. Quantitative-PCR and immunofluorescence staining results indicate that SARS-CoV is capable of replication in HL-CZ cells, and of displaying virus-induced cytopathic effects and increased levels of TNF-α, IL-4 and IL-6 two days post-infection. According to flow cytometry data, the HL-CZ cells also expressed angiotensin converting enzyme 2 (ACE2, a SARS-CoV receptor) and higher levels of the FcγRII receptor. We found that higher concentrations of anti-sera against SARS-CoV neutralized SARS-CoV infection, while highly diluted anti-sera significantly increased SARS-CoV infection and induced higher levels of apoptosis. Results from infectivity assays indicate that SARS-CoV ADE is primarily mediated by diluted antibodies against envelope spike proteins rather than nucleocapsid proteins. We also generated monoclonal antibodies against SARS-CoV spike proteins and observed that most of them promoted SARS-CoV infection. Combined, our results suggest that antibodies against SARS-CoV spike proteins may trigger ADE effects. The data raise new questions regarding a potential SARS-CoV vaccine, while shedding light on mechanisms involved in SARS pathogenesis. |
format | Online Article Text |
id | pubmed-7092860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70928602020-03-25 Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins Wang, Sheng-Fan Tseng, Sung-Pin Yen, Chia-Hung Yang, Jyh-Yuan Tsao, Ching-Han Shen, Chun-Wei Chen, Kuan-Hsuan Liu, Fu-Tong Liu, Wu-Tse Chen, Yi-Ming Arthur Huang, Jason C. Biochem Biophys Res Commun Article The severe acute respiratory syndrome coronavirus (SARS-CoV) still carries the potential for reemergence, therefore efforts are being made to create a vaccine as a prophylactic strategy for control and prevention. Antibody-dependent enhancement (ADE) is a mechanism through which dengue viruses, feline coronaviruses, and HIV viruses take advantage of anti-viral humoral immune responses to infect host target cells. Here we describe our observations of SARS-CoV using ADE to enhance the infectivity of a HL-CZ human promonocyte cell line. Quantitative-PCR and immunofluorescence staining results indicate that SARS-CoV is capable of replication in HL-CZ cells, and of displaying virus-induced cytopathic effects and increased levels of TNF-α, IL-4 and IL-6 two days post-infection. According to flow cytometry data, the HL-CZ cells also expressed angiotensin converting enzyme 2 (ACE2, a SARS-CoV receptor) and higher levels of the FcγRII receptor. We found that higher concentrations of anti-sera against SARS-CoV neutralized SARS-CoV infection, while highly diluted anti-sera significantly increased SARS-CoV infection and induced higher levels of apoptosis. Results from infectivity assays indicate that SARS-CoV ADE is primarily mediated by diluted antibodies against envelope spike proteins rather than nucleocapsid proteins. We also generated monoclonal antibodies against SARS-CoV spike proteins and observed that most of them promoted SARS-CoV infection. Combined, our results suggest that antibodies against SARS-CoV spike proteins may trigger ADE effects. The data raise new questions regarding a potential SARS-CoV vaccine, while shedding light on mechanisms involved in SARS pathogenesis. Elsevier Inc. 2014-08-22 2014-07-26 /pmc/articles/PMC7092860/ /pubmed/25073113 http://dx.doi.org/10.1016/j.bbrc.2014.07.090 Text en Copyright © 2014 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Wang, Sheng-Fan Tseng, Sung-Pin Yen, Chia-Hung Yang, Jyh-Yuan Tsao, Ching-Han Shen, Chun-Wei Chen, Kuan-Hsuan Liu, Fu-Tong Liu, Wu-Tse Chen, Yi-Ming Arthur Huang, Jason C. Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title | Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title_full | Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title_fullStr | Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title_full_unstemmed | Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title_short | Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins |
title_sort | antibody-dependent sars coronavirus infection is mediated by antibodies against spike proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092860/ https://www.ncbi.nlm.nih.gov/pubmed/25073113 http://dx.doi.org/10.1016/j.bbrc.2014.07.090 |
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