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The severe acute respiratory syndrome coronavirus 3a is a novel structural protein
The severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is one of the opening reading frames in the viral genome with no homologue in other known coronaviruses. Expression of the 3a protein has been demonstrated during both in vitro and in vivo infection. Here we present biochemical...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092867/ https://www.ncbi.nlm.nih.gov/pubmed/15781262 http://dx.doi.org/10.1016/j.bbrc.2005.02.153 |
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author | Shen, Shuo Lin, Pi-Shiu Chao, Yu-Chan Zhang, Aihua Yang, Xiaoming Lim, Seng Gee Hong, Wanjin Tan, Yee-Joo |
author_facet | Shen, Shuo Lin, Pi-Shiu Chao, Yu-Chan Zhang, Aihua Yang, Xiaoming Lim, Seng Gee Hong, Wanjin Tan, Yee-Joo |
author_sort | Shen, Shuo |
collection | PubMed |
description | The severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is one of the opening reading frames in the viral genome with no homologue in other known coronaviruses. Expression of the 3a protein has been demonstrated during both in vitro and in vivo infection. Here we present biochemical data to show that 3a is a novel coronavirus structural protein. 3a was detected in virions purified from SARS-CoV infected Vero E6 cells although two truncated products were present predominantly instead of the full-length protein. In Vero E6 cells transiently transfected with a cDNA construct for expressing 3a, a similar cleavage was observed. Furthermore, co-expression of 3a, membrane and envelope proteins using the baculovirus system showed that both full-length and truncated 3a can be assembled into virus-like particles. This is the first report that demonstrated the incorporation of 3a into virion and showed that the SARS-CoV encodes a novel coronavirus structural protein. |
format | Online Article Text |
id | pubmed-7092867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70928672020-03-25 The severe acute respiratory syndrome coronavirus 3a is a novel structural protein Shen, Shuo Lin, Pi-Shiu Chao, Yu-Chan Zhang, Aihua Yang, Xiaoming Lim, Seng Gee Hong, Wanjin Tan, Yee-Joo Biochem Biophys Res Commun Article The severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is one of the opening reading frames in the viral genome with no homologue in other known coronaviruses. Expression of the 3a protein has been demonstrated during both in vitro and in vivo infection. Here we present biochemical data to show that 3a is a novel coronavirus structural protein. 3a was detected in virions purified from SARS-CoV infected Vero E6 cells although two truncated products were present predominantly instead of the full-length protein. In Vero E6 cells transiently transfected with a cDNA construct for expressing 3a, a similar cleavage was observed. Furthermore, co-expression of 3a, membrane and envelope proteins using the baculovirus system showed that both full-length and truncated 3a can be assembled into virus-like particles. This is the first report that demonstrated the incorporation of 3a into virion and showed that the SARS-CoV encodes a novel coronavirus structural protein. Elsevier Inc. 2005-04-29 2005-03-05 /pmc/articles/PMC7092867/ /pubmed/15781262 http://dx.doi.org/10.1016/j.bbrc.2005.02.153 Text en Copyright © 2005 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Shen, Shuo Lin, Pi-Shiu Chao, Yu-Chan Zhang, Aihua Yang, Xiaoming Lim, Seng Gee Hong, Wanjin Tan, Yee-Joo The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title | The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title_full | The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title_fullStr | The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title_full_unstemmed | The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title_short | The severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
title_sort | severe acute respiratory syndrome coronavirus 3a is a novel structural protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092867/ https://www.ncbi.nlm.nih.gov/pubmed/15781262 http://dx.doi.org/10.1016/j.bbrc.2005.02.153 |
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