Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response

Leucine-rich α(2)-glycoprotein (LRG) is a plasma protein in which leucine-rich repeats (LRRs) were first discovered. Although the physiological function of LRG is not known, increases in the serum level of LRG have been reported in various diseases. In this study, we found that LRG was induced by re...

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Autores principales: Shirai, Ryoichi, Hirano, Fumiyasu, Ohkura, Naoki, Ikeda, Kiyoshi, Inoue, Seiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092932/
https://www.ncbi.nlm.nih.gov/pubmed/19324010
http://dx.doi.org/10.1016/j.bbrc.2009.03.104
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author Shirai, Ryoichi
Hirano, Fumiyasu
Ohkura, Naoki
Ikeda, Kiyoshi
Inoue, Seiji
author_facet Shirai, Ryoichi
Hirano, Fumiyasu
Ohkura, Naoki
Ikeda, Kiyoshi
Inoue, Seiji
author_sort Shirai, Ryoichi
collection PubMed
description Leucine-rich α(2)-glycoprotein (LRG) is a plasma protein in which leucine-rich repeats (LRRs) were first discovered. Although the physiological function of LRG is not known, increases in the serum level of LRG have been reported in various diseases. In this study, we found that LRG was induced by recombinant human IL-6 in human hepatoma HepG2 cells. The induction of LRG by IL-6 was up-regulated synergistically with either IL-1β or TNFα in a pattern similar to those for type 1 acute-phase proteins. We also found that lipopolysaccharide (LPS) administered intraperitoneally to mice enhanced dose-dependently the expression of LRG mRNA in the liver as well as those for mouse major acute-phase proteins. These results strongly suggest that LRG was a secretory type 1 acute-phase protein whose expression was up-regulated by the mediator of acute-phase response.
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spelling pubmed-70929322020-03-25 Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response Shirai, Ryoichi Hirano, Fumiyasu Ohkura, Naoki Ikeda, Kiyoshi Inoue, Seiji Biochem Biophys Res Commun Article Leucine-rich α(2)-glycoprotein (LRG) is a plasma protein in which leucine-rich repeats (LRRs) were first discovered. Although the physiological function of LRG is not known, increases in the serum level of LRG have been reported in various diseases. In this study, we found that LRG was induced by recombinant human IL-6 in human hepatoma HepG2 cells. The induction of LRG by IL-6 was up-regulated synergistically with either IL-1β or TNFα in a pattern similar to those for type 1 acute-phase proteins. We also found that lipopolysaccharide (LPS) administered intraperitoneally to mice enhanced dose-dependently the expression of LRG mRNA in the liver as well as those for mouse major acute-phase proteins. These results strongly suggest that LRG was a secretory type 1 acute-phase protein whose expression was up-regulated by the mediator of acute-phase response. Elsevier Inc. 2009-05-15 2009-03-24 /pmc/articles/PMC7092932/ /pubmed/19324010 http://dx.doi.org/10.1016/j.bbrc.2009.03.104 Text en Copyright © 2009 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Shirai, Ryoichi
Hirano, Fumiyasu
Ohkura, Naoki
Ikeda, Kiyoshi
Inoue, Seiji
Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title_full Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title_fullStr Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title_full_unstemmed Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title_short Up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
title_sort up-regulation of the expression of leucine-rich α(2)-glycoprotein in hepatocytes by the mediators of acute-phase response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092932/
https://www.ncbi.nlm.nih.gov/pubmed/19324010
http://dx.doi.org/10.1016/j.bbrc.2009.03.104
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