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Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots

Pesticides, widely used in modern agriculture, could potentially cause environmental pollution and affect human lives. Hence, the development of a highly sensitive sensing element to detect pesticide residues is crucial for food safety and ecosystem protection. Optical methods based on fluorescence...

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Autores principales: Ashrafi Tafreshi, Fatemeh, Fatahi, Zahra, Ghasemi, Seyedeh Fatemeh, Taherian, Amirali, Esfandiari, Neda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092965/
https://www.ncbi.nlm.nih.gov/pubmed/32208468
http://dx.doi.org/10.1371/journal.pone.0230646
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author Ashrafi Tafreshi, Fatemeh
Fatahi, Zahra
Ghasemi, Seyedeh Fatemeh
Taherian, Amirali
Esfandiari, Neda
author_facet Ashrafi Tafreshi, Fatemeh
Fatahi, Zahra
Ghasemi, Seyedeh Fatemeh
Taherian, Amirali
Esfandiari, Neda
author_sort Ashrafi Tafreshi, Fatemeh
collection PubMed
description Pesticides, widely used in modern agriculture, could potentially cause environmental pollution and affect human lives. Hence, the development of a highly sensitive sensing element to detect pesticide residues is crucial for food safety and ecosystem protection. Optical methods based on fluorescence properties provide an ideal approach for screening and quantification of these compounds in different medias including water, plant, and nutritional products. The development of fluorescence emitting carbon dot-based sensors for monitoring pesticides has attracted great attention in recent years. In comparison to other fluorophores, carbon dots have more promising optical features, higher quantum yields and better biocompatibility. This article aims to present a novel fluorescent sensing method of diazinon, glyphosate, and amicarbazone using plant-based carbon dots. A comprehensive characterization of carbon dots obtained from cauliflower was performed by methods including UV-visible, FTIR spectroscopy, fluorometry, AFM, DLS, and zeta sizer. Following this step, carbon dots were used to detect pesticides. The fluorescence quenching property of carbon dots has been utilized to identify detection limit of 0.25, 0.5, and 2 ng ml(-1) for diazinon, amicarbazone, and glyphosate, respectively. Also, real sample study revealed that the detection of pesticides accompanied by our developed nano-sensor is repeatable and accurate. According to carbon dots specificity determination, the prepared nano sensor does not have the potential to identify “bromacil” and “dialen super” pesticides but the other three mentioned pesticides are detectable. The results confirm that synthesized green carbon dots are well qualified for application in food safety and environmental monitoring.
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spelling pubmed-70929652020-04-01 Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots Ashrafi Tafreshi, Fatemeh Fatahi, Zahra Ghasemi, Seyedeh Fatemeh Taherian, Amirali Esfandiari, Neda PLoS One Research Article Pesticides, widely used in modern agriculture, could potentially cause environmental pollution and affect human lives. Hence, the development of a highly sensitive sensing element to detect pesticide residues is crucial for food safety and ecosystem protection. Optical methods based on fluorescence properties provide an ideal approach for screening and quantification of these compounds in different medias including water, plant, and nutritional products. The development of fluorescence emitting carbon dot-based sensors for monitoring pesticides has attracted great attention in recent years. In comparison to other fluorophores, carbon dots have more promising optical features, higher quantum yields and better biocompatibility. This article aims to present a novel fluorescent sensing method of diazinon, glyphosate, and amicarbazone using plant-based carbon dots. A comprehensive characterization of carbon dots obtained from cauliflower was performed by methods including UV-visible, FTIR spectroscopy, fluorometry, AFM, DLS, and zeta sizer. Following this step, carbon dots were used to detect pesticides. The fluorescence quenching property of carbon dots has been utilized to identify detection limit of 0.25, 0.5, and 2 ng ml(-1) for diazinon, amicarbazone, and glyphosate, respectively. Also, real sample study revealed that the detection of pesticides accompanied by our developed nano-sensor is repeatable and accurate. According to carbon dots specificity determination, the prepared nano sensor does not have the potential to identify “bromacil” and “dialen super” pesticides but the other three mentioned pesticides are detectable. The results confirm that synthesized green carbon dots are well qualified for application in food safety and environmental monitoring. Public Library of Science 2020-03-24 /pmc/articles/PMC7092965/ /pubmed/32208468 http://dx.doi.org/10.1371/journal.pone.0230646 Text en © 2020 Ashrafi Tafreshi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ashrafi Tafreshi, Fatemeh
Fatahi, Zahra
Ghasemi, Seyedeh Fatemeh
Taherian, Amirali
Esfandiari, Neda
Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title_full Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title_fullStr Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title_full_unstemmed Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title_short Ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
title_sort ultrasensitive fluorescent detection of pesticides in real sample by using green carbon dots
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092965/
https://www.ncbi.nlm.nih.gov/pubmed/32208468
http://dx.doi.org/10.1371/journal.pone.0230646
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