The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity

The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the...

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Autores principales: Dydecka, Aleksandra, Bloch, Sylwia, Necel, Agnieszka, Topka, Gracja, Węgrzyn, Alicja, Tong, Jinge, Donaldson, Logan W., Węgrzyn, Grzegorz, Nejman-Faleńczyk, Bożena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
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Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093339/
https://www.ncbi.nlm.nih.gov/pubmed/31970620
http://dx.doi.org/10.1007/s11262-020-01734-8
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author Dydecka, Aleksandra
Bloch, Sylwia
Necel, Agnieszka
Topka, Gracja
Węgrzyn, Alicja
Tong, Jinge
Donaldson, Logan W.
Węgrzyn, Grzegorz
Nejman-Faleńczyk, Bożena
author_facet Dydecka, Aleksandra
Bloch, Sylwia
Necel, Agnieszka
Topka, Gracja
Węgrzyn, Alicja
Tong, Jinge
Donaldson, Logan W.
Węgrzyn, Grzegorz
Nejman-Faleńczyk, Bożena
author_sort Dydecka, Aleksandra
collection PubMed
description The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein.
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spelling pubmed-70933392020-03-26 The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity Dydecka, Aleksandra Bloch, Sylwia Necel, Agnieszka Topka, Gracja Węgrzyn, Alicja Tong, Jinge Donaldson, Logan W. Węgrzyn, Grzegorz Nejman-Faleńczyk, Bożena Virus Genes Original Paper The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein. Springer US 2020-01-22 2020 /pmc/articles/PMC7093339/ /pubmed/31970620 http://dx.doi.org/10.1007/s11262-020-01734-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Paper
Dydecka, Aleksandra
Bloch, Sylwia
Necel, Agnieszka
Topka, Gracja
Węgrzyn, Alicja
Tong, Jinge
Donaldson, Logan W.
Węgrzyn, Grzegorz
Nejman-Faleńczyk, Bożena
The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title_full The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title_fullStr The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title_full_unstemmed The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title_short The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
title_sort ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093339/
https://www.ncbi.nlm.nih.gov/pubmed/31970620
http://dx.doi.org/10.1007/s11262-020-01734-8
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