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Nanoparticle corona artefacts derived from specimen preparation of particle suspensions
Progress in the implementation of nanoparticles for therapeutic applications will accelerate with an improved understanding of the interface between nanoparticle surfaces and the media they are dispersed in. We examine this interface by analytical scanning transmission electron microscopy and show t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093507/ https://www.ncbi.nlm.nih.gov/pubmed/32210326 http://dx.doi.org/10.1038/s41598-020-62253-y |
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author | Ilett, Martha Matar, Omar Bamiduro, Faith Sanchez-Segado, Sergio Brydson, Rik Brown, Andy Hondow, Nicole |
author_facet | Ilett, Martha Matar, Omar Bamiduro, Faith Sanchez-Segado, Sergio Brydson, Rik Brown, Andy Hondow, Nicole |
author_sort | Ilett, Martha |
collection | PubMed |
description | Progress in the implementation of nanoparticles for therapeutic applications will accelerate with an improved understanding of the interface between nanoparticle surfaces and the media they are dispersed in. We examine this interface by analytical scanning transmission electron microscopy and show that incorrect specimen preparation or analysis can induce an artefactual, nanoscale, calcium phosphate-rich, amorphous coating on nanoparticles dispersed in cell culture media. We report that this ionic coating can be induced on five different types of nanoparticles (Au, BaTiO(3), ZnO, TiO(2) and Fe(2)O(3)) when specimen preparation causes a significant rise in pH above physiological levels. Such a pH change reduces ionic solubility in the suspending media to permit precipitation of calcium phosphate. Finally, we demonstrate that there is no indication of a calcium-phosphorus-rich coating on BaTiO(3) nanoparticles suspended in culture media when prepared without alteration of the pH of the suspending media and imaged by cryo-STEM. Therefore we recommend that future reports utilising nanoparticles dispersed in cell culture media monitor and report the pH of suspensions during sample preparation. |
format | Online Article Text |
id | pubmed-7093507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70935072020-03-27 Nanoparticle corona artefacts derived from specimen preparation of particle suspensions Ilett, Martha Matar, Omar Bamiduro, Faith Sanchez-Segado, Sergio Brydson, Rik Brown, Andy Hondow, Nicole Sci Rep Article Progress in the implementation of nanoparticles for therapeutic applications will accelerate with an improved understanding of the interface between nanoparticle surfaces and the media they are dispersed in. We examine this interface by analytical scanning transmission electron microscopy and show that incorrect specimen preparation or analysis can induce an artefactual, nanoscale, calcium phosphate-rich, amorphous coating on nanoparticles dispersed in cell culture media. We report that this ionic coating can be induced on five different types of nanoparticles (Au, BaTiO(3), ZnO, TiO(2) and Fe(2)O(3)) when specimen preparation causes a significant rise in pH above physiological levels. Such a pH change reduces ionic solubility in the suspending media to permit precipitation of calcium phosphate. Finally, we demonstrate that there is no indication of a calcium-phosphorus-rich coating on BaTiO(3) nanoparticles suspended in culture media when prepared without alteration of the pH of the suspending media and imaged by cryo-STEM. Therefore we recommend that future reports utilising nanoparticles dispersed in cell culture media monitor and report the pH of suspensions during sample preparation. Nature Publishing Group UK 2020-03-24 /pmc/articles/PMC7093507/ /pubmed/32210326 http://dx.doi.org/10.1038/s41598-020-62253-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ilett, Martha Matar, Omar Bamiduro, Faith Sanchez-Segado, Sergio Brydson, Rik Brown, Andy Hondow, Nicole Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title | Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title_full | Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title_fullStr | Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title_full_unstemmed | Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title_short | Nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
title_sort | nanoparticle corona artefacts derived from specimen preparation of particle suspensions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093507/ https://www.ncbi.nlm.nih.gov/pubmed/32210326 http://dx.doi.org/10.1038/s41598-020-62253-y |
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