Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana

In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diac...

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Autores principales: Takaoka, Yousuke, Miyagawa, Saki, Nakamura, Akinobu, Egoshi, Syusuke, Tsukiji, Shinya, Ueda, Minoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093514/
https://www.ncbi.nlm.nih.gov/pubmed/32210301
http://dx.doi.org/10.1038/s41598-020-62239-w
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author Takaoka, Yousuke
Miyagawa, Saki
Nakamura, Akinobu
Egoshi, Syusuke
Tsukiji, Shinya
Ueda, Minoru
author_facet Takaoka, Yousuke
Miyagawa, Saki
Nakamura, Akinobu
Egoshi, Syusuke
Tsukiji, Shinya
Ueda, Minoru
author_sort Takaoka, Yousuke
collection PubMed
description In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc(2)Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata.
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spelling pubmed-70935142020-03-27 Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana Takaoka, Yousuke Miyagawa, Saki Nakamura, Akinobu Egoshi, Syusuke Tsukiji, Shinya Ueda, Minoru Sci Rep Article In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc(2)Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata. Nature Publishing Group UK 2020-03-24 /pmc/articles/PMC7093514/ /pubmed/32210301 http://dx.doi.org/10.1038/s41598-020-62239-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Takaoka, Yousuke
Miyagawa, Saki
Nakamura, Akinobu
Egoshi, Syusuke
Tsukiji, Shinya
Ueda, Minoru
Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title_full Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title_fullStr Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title_full_unstemmed Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title_short Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana
title_sort hoechst-tagged fluorescein diacetate for the fluorescence imaging-based assessment of stomatal dynamics in arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093514/
https://www.ncbi.nlm.nih.gov/pubmed/32210301
http://dx.doi.org/10.1038/s41598-020-62239-w
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