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Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products
Actinomycete bacteria from marine environments represent a potential source for new antibiotics and anti-tumor drugs. Ten strains belonging to the genus Streptomyces isolated from the marine sponge Antho dichotoma collected at the bottom of the Trondheim fjord (Norway) were screened for antibiotic a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093587/ https://www.ncbi.nlm.nih.gov/pubmed/32256483 http://dx.doi.org/10.3389/fmicb.2020.00437 |
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author | Guerrero-Garzón, Jaime Felipe Zehl, Martin Schneider, Olha Rückert, Christian Busche, Tobias Kalinowski, Jörn Bredholt, Harald Zotchev, Sergey B. |
author_facet | Guerrero-Garzón, Jaime Felipe Zehl, Martin Schneider, Olha Rückert, Christian Busche, Tobias Kalinowski, Jörn Bredholt, Harald Zotchev, Sergey B. |
author_sort | Guerrero-Garzón, Jaime Felipe |
collection | PubMed |
description | Actinomycete bacteria from marine environments represent a potential source for new antibiotics and anti-tumor drugs. Ten strains belonging to the genus Streptomyces isolated from the marine sponge Antho dichotoma collected at the bottom of the Trondheim fjord (Norway) were screened for antibiotic activity. Since only few isolates proved to be bioactive in the conditions tested, we decided to gain an insight into their biosynthetic potential using genome sequencing and analysis. Draft genomes were analyzed for the presence of secondary metabolite biosynthesis gene clusters (BGCs) using antiSMASH software. BGCs specifying both known and potentially novel secondary metabolites were identified, suggesting that these isolates might be sources for new bioactive compounds. The results of this analysis also implied horizontal transfer of several gene clusters between the studied isolates, which was especially evident for the lantibiotic- and thiopeptide-encoding BGCs. The latter implies the significance of particular secondary metabolites for the adaptation of Streptomyces to the spatially enclosed marine environments such as marine sponges. Two bioactive isolates, one showing activity against both yeast and Bacillus subtilis, and one only against yeast were analyzed in details, leading to the identification of cycloheximide, linearmycins, and echinomycins that are presumably responsible for the observed bioactivities. |
format | Online Article Text |
id | pubmed-7093587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70935872020-04-01 Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products Guerrero-Garzón, Jaime Felipe Zehl, Martin Schneider, Olha Rückert, Christian Busche, Tobias Kalinowski, Jörn Bredholt, Harald Zotchev, Sergey B. Front Microbiol Microbiology Actinomycete bacteria from marine environments represent a potential source for new antibiotics and anti-tumor drugs. Ten strains belonging to the genus Streptomyces isolated from the marine sponge Antho dichotoma collected at the bottom of the Trondheim fjord (Norway) were screened for antibiotic activity. Since only few isolates proved to be bioactive in the conditions tested, we decided to gain an insight into their biosynthetic potential using genome sequencing and analysis. Draft genomes were analyzed for the presence of secondary metabolite biosynthesis gene clusters (BGCs) using antiSMASH software. BGCs specifying both known and potentially novel secondary metabolites were identified, suggesting that these isolates might be sources for new bioactive compounds. The results of this analysis also implied horizontal transfer of several gene clusters between the studied isolates, which was especially evident for the lantibiotic- and thiopeptide-encoding BGCs. The latter implies the significance of particular secondary metabolites for the adaptation of Streptomyces to the spatially enclosed marine environments such as marine sponges. Two bioactive isolates, one showing activity against both yeast and Bacillus subtilis, and one only against yeast were analyzed in details, leading to the identification of cycloheximide, linearmycins, and echinomycins that are presumably responsible for the observed bioactivities. Frontiers Media S.A. 2020-03-18 /pmc/articles/PMC7093587/ /pubmed/32256483 http://dx.doi.org/10.3389/fmicb.2020.00437 Text en Copyright © 2020 Guerrero-Garzón, Zehl, Schneider, Rückert, Busche, Kalinowski, Bredholt and Zotchev. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Guerrero-Garzón, Jaime Felipe Zehl, Martin Schneider, Olha Rückert, Christian Busche, Tobias Kalinowski, Jörn Bredholt, Harald Zotchev, Sergey B. Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title | Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title_full | Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title_fullStr | Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title_full_unstemmed | Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title_short | Streptomyces spp. From the Marine Sponge Antho dichotoma: Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products |
title_sort | streptomyces spp. from the marine sponge antho dichotoma: analyses of secondary metabolite biosynthesis gene clusters and some of their products |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093587/ https://www.ncbi.nlm.nih.gov/pubmed/32256483 http://dx.doi.org/10.3389/fmicb.2020.00437 |
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