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Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently

The discovery of new members of the Tc1/mariner superfamily of transposons is expected based on the increasing availability of genome sequencing data. Here, we identified a new DD35E family termed Traveler (TR). Phylogenetic analyses of its DDE domain and full-length transposase showed that, althoug...

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Autores principales: Zong, Wencheng, Gao, Bo, Diaby, Mohamed, Shen, Dan, Wang, Saisai, Wang, Yali, Sang, Yatong, Chen, Cai, Wang, Xiaoyan, Song, Chengyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093834/
https://www.ncbi.nlm.nih.gov/pubmed/32068835
http://dx.doi.org/10.1093/gbe/evaa034
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author Zong, Wencheng
Gao, Bo
Diaby, Mohamed
Shen, Dan
Wang, Saisai
Wang, Yali
Sang, Yatong
Chen, Cai
Wang, Xiaoyan
Song, Chengyi
author_facet Zong, Wencheng
Gao, Bo
Diaby, Mohamed
Shen, Dan
Wang, Saisai
Wang, Yali
Sang, Yatong
Chen, Cai
Wang, Xiaoyan
Song, Chengyi
author_sort Zong, Wencheng
collection PubMed
description The discovery of new members of the Tc1/mariner superfamily of transposons is expected based on the increasing availability of genome sequencing data. Here, we identified a new DD35E family termed Traveler (TR). Phylogenetic analyses of its DDE domain and full-length transposase showed that, although TR formed a monophyletic clade, it exhibited the highest sequence identity and closest phylogenetic relationship with DD34E/Tc1. This family displayed a very restricted taxonomic distribution in the animal kingdom and was only detected in ray-finned fish, anura, and squamata, including 91 vertebrate species. The structural organization of TRs was highly conserved across different classes of animals. Most intact TR transposons had a length of ∼1.5 kb (range 1,072–2,191 bp) and harbored a single open reading frame encoding a transposase of ∼340 aa (range 304–350 aa) flanked by two short-terminal inverted repeats (13–68 bp). Several conserved motifs, including two helix-turn-helix motifs, a GRPR motif, a nuclear localization sequence, and a DDE domain, were also identified in TR transposases. This study also demonstrated the presence of horizontal transfer events of TRs in vertebrates, whereas the average sequence identities and the evolutionary dynamics of TR elements across species and clusters strongly indicated that the TR family invaded the vertebrate lineage very recently and that some of these elements may be currently active, combining the intact TR copies in multiple lineages of vertebrates. These data will contribute to the understanding of the evolutionary history of Tc1/mariner transposons and that of their hosts.
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spelling pubmed-70938342020-03-30 Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently Zong, Wencheng Gao, Bo Diaby, Mohamed Shen, Dan Wang, Saisai Wang, Yali Sang, Yatong Chen, Cai Wang, Xiaoyan Song, Chengyi Genome Biol Evol Research Article The discovery of new members of the Tc1/mariner superfamily of transposons is expected based on the increasing availability of genome sequencing data. Here, we identified a new DD35E family termed Traveler (TR). Phylogenetic analyses of its DDE domain and full-length transposase showed that, although TR formed a monophyletic clade, it exhibited the highest sequence identity and closest phylogenetic relationship with DD34E/Tc1. This family displayed a very restricted taxonomic distribution in the animal kingdom and was only detected in ray-finned fish, anura, and squamata, including 91 vertebrate species. The structural organization of TRs was highly conserved across different classes of animals. Most intact TR transposons had a length of ∼1.5 kb (range 1,072–2,191 bp) and harbored a single open reading frame encoding a transposase of ∼340 aa (range 304–350 aa) flanked by two short-terminal inverted repeats (13–68 bp). Several conserved motifs, including two helix-turn-helix motifs, a GRPR motif, a nuclear localization sequence, and a DDE domain, were also identified in TR transposases. This study also demonstrated the presence of horizontal transfer events of TRs in vertebrates, whereas the average sequence identities and the evolutionary dynamics of TR elements across species and clusters strongly indicated that the TR family invaded the vertebrate lineage very recently and that some of these elements may be currently active, combining the intact TR copies in multiple lineages of vertebrates. These data will contribute to the understanding of the evolutionary history of Tc1/mariner transposons and that of their hosts. Oxford University Press 2020-02-18 /pmc/articles/PMC7093834/ /pubmed/32068835 http://dx.doi.org/10.1093/gbe/evaa034 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zong, Wencheng
Gao, Bo
Diaby, Mohamed
Shen, Dan
Wang, Saisai
Wang, Yali
Sang, Yatong
Chen, Cai
Wang, Xiaoyan
Song, Chengyi
Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title_full Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title_fullStr Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title_full_unstemmed Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title_short Traveler, a New DD35E Family of Tc1/Mariner Transposons, Invaded Vertebrates Very Recently
title_sort traveler, a new dd35e family of tc1/mariner transposons, invaded vertebrates very recently
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093834/
https://www.ncbi.nlm.nih.gov/pubmed/32068835
http://dx.doi.org/10.1093/gbe/evaa034
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