Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil

INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of southern Brazil....

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Autores principales: Mendes, Josiara Furtado, Poester, Vanice Rodrigues, Groll, Andrea Von, Meireles, Mário Carlos Araújo, Xavier, Melissa Orzechowski
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094035/
http://dx.doi.org/10.1590/0037-8682-0172-2019
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author Mendes, Josiara Furtado
Poester, Vanice Rodrigues
Groll, Andrea Von
Meireles, Mário Carlos Araújo
Xavier, Melissa Orzechowski
author_facet Mendes, Josiara Furtado
Poester, Vanice Rodrigues
Groll, Andrea Von
Meireles, Mário Carlos Araújo
Xavier, Melissa Orzechowski
author_sort Mendes, Josiara Furtado
collection PubMed
description INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of southern Brazil. METHODS: Sixteen soil samples were submitted to nested-PCR and the amplicons of a representative number of positive samples were sequenced. RESULTS: Paracoccidioides spp. DNA was found in 44% of samples. Four DNA amplicons were sequenced, showing 100% homology with P. brasiliensis. CONCLUSIONS: The southern Brazilian urban population is commonly exposed to the Paracoccidioides fungus.
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spelling pubmed-70940352020-03-25 Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil Mendes, Josiara Furtado Poester, Vanice Rodrigues Groll, Andrea Von Meireles, Mário Carlos Araújo Xavier, Melissa Orzechowski Rev Soc Bras Med Trop Short Communication INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of Paracoccidioides spp. DNA in soil samples from an urban area of southern Brazil. METHODS: Sixteen soil samples were submitted to nested-PCR and the amplicons of a representative number of positive samples were sequenced. RESULTS: Paracoccidioides spp. DNA was found in 44% of samples. Four DNA amplicons were sequenced, showing 100% homology with P. brasiliensis. CONCLUSIONS: The southern Brazilian urban population is commonly exposed to the Paracoccidioides fungus. Sociedade Brasileira de Medicina Tropical - SBMT 2020-03-16 /pmc/articles/PMC7094035/ http://dx.doi.org/10.1590/0037-8682-0172-2019 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Mendes, Josiara Furtado
Poester, Vanice Rodrigues
Groll, Andrea Von
Meireles, Mário Carlos Araújo
Xavier, Melissa Orzechowski
Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title_full Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title_fullStr Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title_full_unstemmed Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title_short Molecular detection of Paracoccidioides in soil from an urban area of southern Brazil
title_sort molecular detection of paracoccidioides in soil from an urban area of southern brazil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094035/
http://dx.doi.org/10.1590/0037-8682-0172-2019
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