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New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA

INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic an...

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Detalles Bibliográficos
Autores principales: Julio, Rafael Guillermo Villarreal, Agudelo-Flórez, Piedad, López, Juan Álvaro, Sánchez, Ronald Guillermo Peláez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094048/
https://www.ncbi.nlm.nih.gov/pubmed/32187337
http://dx.doi.org/10.1590/0037-8682-0333-2019
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author Julio, Rafael Guillermo Villarreal
Agudelo-Flórez, Piedad
López, Juan Álvaro
Sánchez, Ronald Guillermo Peláez
author_facet Julio, Rafael Guillermo Villarreal
Agudelo-Flórez, Piedad
López, Juan Álvaro
Sánchez, Ronald Guillermo Peláez
author_sort Julio, Rafael Guillermo Villarreal
collection PubMed
description INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. RESULTS: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species. CONCLUSIONS: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.
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spelling pubmed-70940482020-03-25 New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA Julio, Rafael Guillermo Villarreal Agudelo-Flórez, Piedad López, Juan Álvaro Sánchez, Ronald Guillermo Peláez Rev Soc Bras Med Trop Short Communication INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. RESULTS: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species. CONCLUSIONS: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples. Sociedade Brasileira de Medicina Tropical - SBMT 2020-03-16 /pmc/articles/PMC7094048/ /pubmed/32187337 http://dx.doi.org/10.1590/0037-8682-0333-2019 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Julio, Rafael Guillermo Villarreal
Agudelo-Flórez, Piedad
López, Juan Álvaro
Sánchez, Ronald Guillermo Peláez
New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_full New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_fullStr New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_full_unstemmed New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_short New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_sort new molecular target for the phylogenetic identification of leptospira species directly from clinical samples: an alternative gene to 16s rrna
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094048/
https://www.ncbi.nlm.nih.gov/pubmed/32187337
http://dx.doi.org/10.1590/0037-8682-0333-2019
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