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Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection
The genome of Arabidopsis encodes more than 60 mitogen-activated protein kinase kinase (MAPKK) kinases (MAPKKKs); however, the functions of most MAPKKKs and their downstream MAPKKs are largely unknown. Here, MAPKKK δ-1 (MKD1), a novel Raf-like MAPKKK, was isolated from Arabidopsis as a subunit of a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094076/ https://www.ncbi.nlm.nih.gov/pubmed/31844896 http://dx.doi.org/10.1093/jxb/erz556 |
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author | Asano, Tomoya Nguyen, Thi Hang-Ni Yasuda, Michiko Sidiq, Yasir Nishimura, Kohji Nakashita, Hideo Nishiuchi, Takumi |
author_facet | Asano, Tomoya Nguyen, Thi Hang-Ni Yasuda, Michiko Sidiq, Yasir Nishimura, Kohji Nakashita, Hideo Nishiuchi, Takumi |
author_sort | Asano, Tomoya |
collection | PubMed |
description | The genome of Arabidopsis encodes more than 60 mitogen-activated protein kinase kinase (MAPKK) kinases (MAPKKKs); however, the functions of most MAPKKKs and their downstream MAPKKs are largely unknown. Here, MAPKKK δ-1 (MKD1), a novel Raf-like MAPKKK, was isolated from Arabidopsis as a subunit of a complex including the transcription factor AtNFXL1, which is involved in the trichothecene phytotoxin response and in disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). A MKD1-dependent cascade positively regulates disease resistance against PstDC3000 and the trichothecene mycotoxin-producing fungal pathogen Fusarium sporotrichioides. MKD1 expression was induced by trichothecenes derived from Fusarium species. MKD1 directly interacted with MKK1 and MKK5 in vivo, and phosphorylated MKK1 and MKK5 in vitro. Correspondingly, mkk1 mutants and MKK5RNAi transgenic plants showed enhanced susceptibility to F. sporotrichioides. MKD1 was required for full activation of two MAPKs (MPK3 and MPK6) by the T-2 toxin and flg22. Finally, quantitative phosphoproteomics suggested that an MKD1-dependent cascade controlled phosphorylation of a disease resistance protein, SUMO, and a mycotoxin-detoxifying enzyme. Our findings suggest that the MKD1–MKK1/MKK5–MPK3/MPK6-dependent signaling cascade is involved in the full immune responses against both bacterial and fungal infection. |
format | Online Article Text |
id | pubmed-7094076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70940762020-03-30 Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection Asano, Tomoya Nguyen, Thi Hang-Ni Yasuda, Michiko Sidiq, Yasir Nishimura, Kohji Nakashita, Hideo Nishiuchi, Takumi J Exp Bot Research Papers The genome of Arabidopsis encodes more than 60 mitogen-activated protein kinase kinase (MAPKK) kinases (MAPKKKs); however, the functions of most MAPKKKs and their downstream MAPKKs are largely unknown. Here, MAPKKK δ-1 (MKD1), a novel Raf-like MAPKKK, was isolated from Arabidopsis as a subunit of a complex including the transcription factor AtNFXL1, which is involved in the trichothecene phytotoxin response and in disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). A MKD1-dependent cascade positively regulates disease resistance against PstDC3000 and the trichothecene mycotoxin-producing fungal pathogen Fusarium sporotrichioides. MKD1 expression was induced by trichothecenes derived from Fusarium species. MKD1 directly interacted with MKK1 and MKK5 in vivo, and phosphorylated MKK1 and MKK5 in vitro. Correspondingly, mkk1 mutants and MKK5RNAi transgenic plants showed enhanced susceptibility to F. sporotrichioides. MKD1 was required for full activation of two MAPKs (MPK3 and MPK6) by the T-2 toxin and flg22. Finally, quantitative phosphoproteomics suggested that an MKD1-dependent cascade controlled phosphorylation of a disease resistance protein, SUMO, and a mycotoxin-detoxifying enzyme. Our findings suggest that the MKD1–MKK1/MKK5–MPK3/MPK6-dependent signaling cascade is involved in the full immune responses against both bacterial and fungal infection. Oxford University Press 2020-03-25 2019-12-17 /pmc/articles/PMC7094076/ /pubmed/31844896 http://dx.doi.org/10.1093/jxb/erz556 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Asano, Tomoya Nguyen, Thi Hang-Ni Yasuda, Michiko Sidiq, Yasir Nishimura, Kohji Nakashita, Hideo Nishiuchi, Takumi Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title | Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title_full | Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title_fullStr | Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title_full_unstemmed | Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title_short | Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection |
title_sort | arabidopsis mapkkk δ-1 is required for full immunity against bacterial and fungal infection |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094076/ https://www.ncbi.nlm.nih.gov/pubmed/31844896 http://dx.doi.org/10.1093/jxb/erz556 |
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