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The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094078/ https://www.ncbi.nlm.nih.gov/pubmed/31872214 http://dx.doi.org/10.1093/jxb/erz564 |
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author | An, Yi Zhou, Yangyan Han, Xiao Shen, Chao Wang, Shu Liu, Chao Yin, Weilun Xia, Xinli |
author_facet | An, Yi Zhou, Yangyan Han, Xiao Shen, Chao Wang, Shu Liu, Chao Yin, Weilun Xia, Xinli |
author_sort | An, Yi |
collection | PubMed |
description | GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes were classified into four subfamilies based on gene structure and phylogenetic relationships. Predicted cis-elements suggested potential roles of poplar GATA genes in light, phytohormone, development, and stress responses. A poplar GATA gene, PdGATA19/PdGNC (GATA nitrate-inducible carbon-metabolism-involved), was identified from a fast growing poplar clone. PdGNC expression was significantly up-regulated in leaves under both high (50 mM) and low (0.2 mM) nitrate concentrations. The CRISPR/Cas9-mediated mutant crispr-GNC showed severely retarded growth and enhanced secondary xylem differentiation. PdGNC-overexpressing transformants exhibited 25–30% faster growth, 20–28% higher biomass accumulation, and ~25% increase in chlorophyll content, photosynthetic rate, and plant height, compared with the wild type. Transcriptomic analysis showed that PdGNC was involved in photosynthetic electron transfer and carbon assimilation in the leaf, cell division and carbohydrate utilization in the stem, and nitrogen uptake in the root. These data indicated that PdGNC plays a crucial role in plant growth and is potentially useful in tree molecular breeding. |
format | Online Article Text |
id | pubmed-7094078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70940782020-03-30 The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar An, Yi Zhou, Yangyan Han, Xiao Shen, Chao Wang, Shu Liu, Chao Yin, Weilun Xia, Xinli J Exp Bot Research Papers GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes were classified into four subfamilies based on gene structure and phylogenetic relationships. Predicted cis-elements suggested potential roles of poplar GATA genes in light, phytohormone, development, and stress responses. A poplar GATA gene, PdGATA19/PdGNC (GATA nitrate-inducible carbon-metabolism-involved), was identified from a fast growing poplar clone. PdGNC expression was significantly up-regulated in leaves under both high (50 mM) and low (0.2 mM) nitrate concentrations. The CRISPR/Cas9-mediated mutant crispr-GNC showed severely retarded growth and enhanced secondary xylem differentiation. PdGNC-overexpressing transformants exhibited 25–30% faster growth, 20–28% higher biomass accumulation, and ~25% increase in chlorophyll content, photosynthetic rate, and plant height, compared with the wild type. Transcriptomic analysis showed that PdGNC was involved in photosynthetic electron transfer and carbon assimilation in the leaf, cell division and carbohydrate utilization in the stem, and nitrogen uptake in the root. These data indicated that PdGNC plays a crucial role in plant growth and is potentially useful in tree molecular breeding. Oxford University Press 2020-03-25 2019-12-24 /pmc/articles/PMC7094078/ /pubmed/31872214 http://dx.doi.org/10.1093/jxb/erz564 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers An, Yi Zhou, Yangyan Han, Xiao Shen, Chao Wang, Shu Liu, Chao Yin, Weilun Xia, Xinli The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title | The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title_full | The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title_fullStr | The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title_full_unstemmed | The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title_short | The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar |
title_sort | gata transcription factor gnc plays an important role in photosynthesis and growth in poplar |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094078/ https://www.ncbi.nlm.nih.gov/pubmed/31872214 http://dx.doi.org/10.1093/jxb/erz564 |
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