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The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar

GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes we...

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Autores principales: An, Yi, Zhou, Yangyan, Han, Xiao, Shen, Chao, Wang, Shu, Liu, Chao, Yin, Weilun, Xia, Xinli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094078/
https://www.ncbi.nlm.nih.gov/pubmed/31872214
http://dx.doi.org/10.1093/jxb/erz564
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author An, Yi
Zhou, Yangyan
Han, Xiao
Shen, Chao
Wang, Shu
Liu, Chao
Yin, Weilun
Xia, Xinli
author_facet An, Yi
Zhou, Yangyan
Han, Xiao
Shen, Chao
Wang, Shu
Liu, Chao
Yin, Weilun
Xia, Xinli
author_sort An, Yi
collection PubMed
description GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes were classified into four subfamilies based on gene structure and phylogenetic relationships. Predicted cis-elements suggested potential roles of poplar GATA genes in light, phytohormone, development, and stress responses. A poplar GATA gene, PdGATA19/PdGNC (GATA nitrate-inducible carbon-metabolism-involved), was identified from a fast growing poplar clone. PdGNC expression was significantly up-regulated in leaves under both high (50 mM) and low (0.2 mM) nitrate concentrations. The CRISPR/Cas9-mediated mutant crispr-GNC showed severely retarded growth and enhanced secondary xylem differentiation. PdGNC-overexpressing transformants exhibited 25–30% faster growth, 20–28% higher biomass accumulation, and ~25% increase in chlorophyll content, photosynthetic rate, and plant height, compared with the wild type. Transcriptomic analysis showed that PdGNC was involved in photosynthetic electron transfer and carbon assimilation in the leaf, cell division and carbohydrate utilization in the stem, and nitrogen uptake in the root. These data indicated that PdGNC plays a crucial role in plant growth and is potentially useful in tree molecular breeding.
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spelling pubmed-70940782020-03-30 The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar An, Yi Zhou, Yangyan Han, Xiao Shen, Chao Wang, Shu Liu, Chao Yin, Weilun Xia, Xinli J Exp Bot Research Papers GATA transcription factors are involved in the regulation of diverse growth processes and environmental responses in Arabidopsis and rice. In this study, we conducted a comprehensive bioinformatic survey of the GATA family in the woody perennial Populus trichocarpa. Thirty-nine Populus GATA genes were classified into four subfamilies based on gene structure and phylogenetic relationships. Predicted cis-elements suggested potential roles of poplar GATA genes in light, phytohormone, development, and stress responses. A poplar GATA gene, PdGATA19/PdGNC (GATA nitrate-inducible carbon-metabolism-involved), was identified from a fast growing poplar clone. PdGNC expression was significantly up-regulated in leaves under both high (50 mM) and low (0.2 mM) nitrate concentrations. The CRISPR/Cas9-mediated mutant crispr-GNC showed severely retarded growth and enhanced secondary xylem differentiation. PdGNC-overexpressing transformants exhibited 25–30% faster growth, 20–28% higher biomass accumulation, and ~25% increase in chlorophyll content, photosynthetic rate, and plant height, compared with the wild type. Transcriptomic analysis showed that PdGNC was involved in photosynthetic electron transfer and carbon assimilation in the leaf, cell division and carbohydrate utilization in the stem, and nitrogen uptake in the root. These data indicated that PdGNC plays a crucial role in plant growth and is potentially useful in tree molecular breeding. Oxford University Press 2020-03-25 2019-12-24 /pmc/articles/PMC7094078/ /pubmed/31872214 http://dx.doi.org/10.1093/jxb/erz564 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
An, Yi
Zhou, Yangyan
Han, Xiao
Shen, Chao
Wang, Shu
Liu, Chao
Yin, Weilun
Xia, Xinli
The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title_full The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title_fullStr The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title_full_unstemmed The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title_short The GATA transcription factor GNC plays an important role in photosynthesis and growth in poplar
title_sort gata transcription factor gnc plays an important role in photosynthesis and growth in poplar
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094078/
https://www.ncbi.nlm.nih.gov/pubmed/31872214
http://dx.doi.org/10.1093/jxb/erz564
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