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A malectin domain kinesin functions in pollen and seed development in Arabidopsis
The kinesin family is greatly expanded in plants compared with animals and, with more than a third up-regulated in expression during cell division, it has been suggested that this expansion facilitated complex plant-specific cytoskeletal rearrangements. The cell cycle-regulated kinesins include two...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094084/ https://www.ncbi.nlm.nih.gov/pubmed/31950166 http://dx.doi.org/10.1093/jxb/eraa023 |
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author | Galindo-Trigo, Sergio Grand, Thomas M Voigt, Christian A Smith, Lisa M |
author_facet | Galindo-Trigo, Sergio Grand, Thomas M Voigt, Christian A Smith, Lisa M |
author_sort | Galindo-Trigo, Sergio |
collection | PubMed |
description | The kinesin family is greatly expanded in plants compared with animals and, with more than a third up-regulated in expression during cell division, it has been suggested that this expansion facilitated complex plant-specific cytoskeletal rearrangements. The cell cycle-regulated kinesins include two with an N-terminal malectin domain, a protein domain that has been shown to bind polysaccharides and peptides when found extracellularly in receptor-like kinases. Although malectin domain kinesins are evolutionarily deep rooted, their function in plants remains unclear. Here we show that loss of MALECTIN DOMAIN KINESIN 2 (MDKIN2) results in stochastic developmental defects in pollen, embryo, and endosperm. High rates of seed abnormalities and abortion occur in mdkin2 mutants through a partial maternal effect. No additive effect or additional developmental defects were noted in mdkin1 mdkin2 double mutants. MDKIN2 is expressed in regions of cell division throughout the plant. Subcellular localization of MDKIN2 indicates a role in cell division, with a possible secondary function in the nuclei. Our results reveal a non-essential but important role for a malectin domain kinesin during development in plants. |
format | Online Article Text |
id | pubmed-7094084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70940842020-03-30 A malectin domain kinesin functions in pollen and seed development in Arabidopsis Galindo-Trigo, Sergio Grand, Thomas M Voigt, Christian A Smith, Lisa M J Exp Bot Research Papers The kinesin family is greatly expanded in plants compared with animals and, with more than a third up-regulated in expression during cell division, it has been suggested that this expansion facilitated complex plant-specific cytoskeletal rearrangements. The cell cycle-regulated kinesins include two with an N-terminal malectin domain, a protein domain that has been shown to bind polysaccharides and peptides when found extracellularly in receptor-like kinases. Although malectin domain kinesins are evolutionarily deep rooted, their function in plants remains unclear. Here we show that loss of MALECTIN DOMAIN KINESIN 2 (MDKIN2) results in stochastic developmental defects in pollen, embryo, and endosperm. High rates of seed abnormalities and abortion occur in mdkin2 mutants through a partial maternal effect. No additive effect or additional developmental defects were noted in mdkin1 mdkin2 double mutants. MDKIN2 is expressed in regions of cell division throughout the plant. Subcellular localization of MDKIN2 indicates a role in cell division, with a possible secondary function in the nuclei. Our results reveal a non-essential but important role for a malectin domain kinesin during development in plants. Oxford University Press 2020-03-25 2020-01-17 /pmc/articles/PMC7094084/ /pubmed/31950166 http://dx.doi.org/10.1093/jxb/eraa023 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Galindo-Trigo, Sergio Grand, Thomas M Voigt, Christian A Smith, Lisa M A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title | A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title_full | A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title_fullStr | A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title_full_unstemmed | A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title_short | A malectin domain kinesin functions in pollen and seed development in Arabidopsis |
title_sort | malectin domain kinesin functions in pollen and seed development in arabidopsis |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094084/ https://www.ncbi.nlm.nih.gov/pubmed/31950166 http://dx.doi.org/10.1093/jxb/eraa023 |
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