Structure and function study of paramyxovirus fusion protein heptad repeat peptides

Paramyxovirus might adopt a molecular mechanism of membrane fusion similar to that of other class I viruses in which the heptad repeat (HR) regions of fusion protein (F) HR1 and HR2 form a six-helix bundle structure inducing membrane fusion. In this study, we examined the structure and function of H...

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Detalles Bibliográficos
Autores principales: Wang, Xiao-Jia, Bai, Ya-Duo, Zhang, Guo-Zhong, Zhao, Ji-Xun, Wang, Ming, Gao, George F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094221/
https://www.ncbi.nlm.nih.gov/pubmed/15797244
http://dx.doi.org/10.1016/j.abb.2005.02.004
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author Wang, Xiao-Jia
Bai, Ya-Duo
Zhang, Guo-Zhong
Zhao, Ji-Xun
Wang, Ming
Gao, George F.
author_facet Wang, Xiao-Jia
Bai, Ya-Duo
Zhang, Guo-Zhong
Zhao, Ji-Xun
Wang, Ming
Gao, George F.
author_sort Wang, Xiao-Jia
collection PubMed
description Paramyxovirus might adopt a molecular mechanism of membrane fusion similar to that of other class I viruses in which the heptad repeat (HR) regions of fusion protein (F) HR1 and HR2 form a six-helix bundle structure inducing membrane fusion. In this study, we examined the structure and function of HR1 and HR2 from the avian paramyxovirus-2 (APMV-2) F protein. The study showed that APMV-2 HR1 and HR2 formed a stable six-helix bundle. Only a soluble APMV-2 HR2 peptide showed potent and specific virus-cell fusion inhibition activity. Cross-inhibiting activity with APMV-1 (Newcastle disease virus, NDV) was not found. A possible mechanism of membrane fusion inhibition by the paramyxovirus HR2 peptide is discussed.
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spelling pubmed-70942212020-03-25 Structure and function study of paramyxovirus fusion protein heptad repeat peptides Wang, Xiao-Jia Bai, Ya-Duo Zhang, Guo-Zhong Zhao, Ji-Xun Wang, Ming Gao, George F. Arch Biochem Biophys Article Paramyxovirus might adopt a molecular mechanism of membrane fusion similar to that of other class I viruses in which the heptad repeat (HR) regions of fusion protein (F) HR1 and HR2 form a six-helix bundle structure inducing membrane fusion. In this study, we examined the structure and function of HR1 and HR2 from the avian paramyxovirus-2 (APMV-2) F protein. The study showed that APMV-2 HR1 and HR2 formed a stable six-helix bundle. Only a soluble APMV-2 HR2 peptide showed potent and specific virus-cell fusion inhibition activity. Cross-inhibiting activity with APMV-1 (Newcastle disease virus, NDV) was not found. A possible mechanism of membrane fusion inhibition by the paramyxovirus HR2 peptide is discussed. Published by Elsevier Inc. 2005-04-15 2005-02-09 /pmc/articles/PMC7094221/ /pubmed/15797244 http://dx.doi.org/10.1016/j.abb.2005.02.004 Text en Copyright © 2005 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wang, Xiao-Jia
Bai, Ya-Duo
Zhang, Guo-Zhong
Zhao, Ji-Xun
Wang, Ming
Gao, George F.
Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title_full Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title_fullStr Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title_full_unstemmed Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title_short Structure and function study of paramyxovirus fusion protein heptad repeat peptides
title_sort structure and function study of paramyxovirus fusion protein heptad repeat peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094221/
https://www.ncbi.nlm.nih.gov/pubmed/15797244
http://dx.doi.org/10.1016/j.abb.2005.02.004
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