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Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein

It has been shown that severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS‐CoV M protein using the baculovirus/insect cell expression system, however, we found that the expr...

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Autores principales: Lai, Chia-Wei, Chan, Zun-Ren, Yang, Ding-Gang, Lo, Wen-Hsin, Lai, Yiu-Kay, Chang, Margaret Dah-Tsyr, Hu, Yu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094299/
https://www.ncbi.nlm.nih.gov/pubmed/16797548
http://dx.doi.org/10.1016/j.febslet.2006.06.003
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author Lai, Chia-Wei
Chan, Zun-Ren
Yang, Ding-Gang
Lo, Wen-Hsin
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
author_facet Lai, Chia-Wei
Chan, Zun-Ren
Yang, Ding-Gang
Lo, Wen-Hsin
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
author_sort Lai, Chia-Wei
collection PubMed
description It has been shown that severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS‐CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS‐CoV M protein in animal cells and possible implications are discussed.
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spelling pubmed-70942992020-03-25 Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein Lai, Chia-Wei Chan, Zun-Ren Yang, Ding-Gang Lo, Wen-Hsin Lai, Yiu-Kay Chang, Margaret Dah-Tsyr Hu, Yu-Chen FEBS Lett Short Communications It has been shown that severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) 3a and 7a proteins, but not membrane (M) protein, induce apoptosis in mammalian cells. Upon expression of SARS‐CoV M protein using the baculovirus/insect cell expression system, however, we found that the expressed M protein triggered accelerated apoptosis in insect cells, as characterized by rapid cell death, elevated cytotoxicity, cell shrinkage, nuclear condensation and DNA fragmentation. Conversely, the M protein expressed in mammalian cells did not induce apoptosis. This is the first report describing the induction of apoptosis by SARS‐CoV M protein in animal cells and possible implications are discussed. John Wiley and Sons Inc. 2006-07-10 2006-06-14 /pmc/articles/PMC7094299/ /pubmed/16797548 http://dx.doi.org/10.1016/j.febslet.2006.06.003 Text en FEBS Letters 580 (2006) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Lai, Chia-Wei
Chan, Zun-Ren
Yang, Ding-Gang
Lo, Wen-Hsin
Lai, Yiu-Kay
Chang, Margaret Dah-Tsyr
Hu, Yu-Chen
Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title_full Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title_fullStr Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title_full_unstemmed Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title_short Accelerated induction of apoptosis in insect cells by baculovirus‐expressed SARS‐CoV membrane protein
title_sort accelerated induction of apoptosis in insect cells by baculovirus‐expressed sars‐cov membrane protein
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094299/
https://www.ncbi.nlm.nih.gov/pubmed/16797548
http://dx.doi.org/10.1016/j.febslet.2006.06.003
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