The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization

The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells...

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Detalles Bibliográficos
Autores principales: Geng, Hua, Liu, Yuet-Man, Chan, Wai-Sing, Lo, Anthony Wing-Ip, Au, Deborah Mun-Yee, Waye, Mary Miu-Yee, Ho, Yuan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2005
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094358/
https://www.ncbi.nlm.nih.gov/pubmed/16310783
http://dx.doi.org/10.1016/j.febslet.2005.11.007
Descripción
Sumario:The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

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