The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization

The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells...

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Autores principales: Geng, Hua, Liu, Yuet-Man, Chan, Wai-Sing, Lo, Anthony Wing-Ip, Au, Deborah Mun-Yee, Waye, Mary Miu-Yee, Ho, Yuan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2005
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094358/
https://www.ncbi.nlm.nih.gov/pubmed/16310783
http://dx.doi.org/10.1016/j.febslet.2005.11.007
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author Geng, Hua
Liu, Yuet-Man
Chan, Wai-Sing
Lo, Anthony Wing-Ip
Au, Deborah Mun-Yee
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
author_facet Geng, Hua
Liu, Yuet-Man
Chan, Wai-Sing
Lo, Anthony Wing-Ip
Au, Deborah Mun-Yee
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
author_sort Geng, Hua
collection PubMed
description The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.
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spelling pubmed-70943582020-03-25 The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization Geng, Hua Liu, Yuet-Man Chan, Wai-Sing Lo, Anthony Wing-Ip Au, Deborah Mun-Yee Waye, Mary Miu-Yee Ho, Yuan-Yuan FEBS Lett Short Communications The SARS‐CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS‐CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis. John Wiley and Sons Inc. 2005-12-19 2005-11-21 /pmc/articles/PMC7094358/ /pubmed/16310783 http://dx.doi.org/10.1016/j.febslet.2005.11.007 Text en FEBS Letters 579 (2005) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Geng, Hua
Liu, Yuet-Man
Chan, Wai-Sing
Lo, Anthony Wing-Ip
Au, Deborah Mun-Yee
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title_full The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title_fullStr The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title_full_unstemmed The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title_short The putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: Expression and functional characterization
title_sort putative protein 6 of the severe acute respiratory syndrome‐associated coronavirus: expression and functional characterization
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094358/
https://www.ncbi.nlm.nih.gov/pubmed/16310783
http://dx.doi.org/10.1016/j.febslet.2005.11.007
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