Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells

The pro‐apoptotic properties of severe acute respiratory syndrome coronavirus (SARS‐CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus‐mediate...

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Detalles Bibliográficos
Autores principales: Chow, Ken Y.C., Yeung, Yin Shan, Hon, Chung Chau, Zeng, Fanya, Law, Ka Man, Leung, Frederick C.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2005
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094440/
https://www.ncbi.nlm.nih.gov/pubmed/16310778
http://dx.doi.org/10.1016/j.febslet.2005.10.065
Descripción
Sumario:The pro‐apoptotic properties of severe acute respiratory syndrome coronavirus (SARS‐CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus‐mediated over‐expression of SARS‐CoV spike (S) protein and its C‐terminal domain (S2) induce apoptosis in Vero E6 cells in a time‐ and dosage‐dependent manner, whereas the expression of its N‐terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS‐CoV induced apoptosis and the molecular pathogenesis of SARS.

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