Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells

The pro‐apoptotic properties of severe acute respiratory syndrome coronavirus (SARS‐CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus‐mediate...

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Detalles Bibliográficos
Autores principales: Chow, Ken Y.C., Yeung, Yin Shan, Hon, Chung Chau, Zeng, Fanya, Law, Ka Man, Leung, Frederick C.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2005
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094440/
https://www.ncbi.nlm.nih.gov/pubmed/16310778
http://dx.doi.org/10.1016/j.febslet.2005.10.065
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author Chow, Ken Y.C.
Yeung, Yin Shan
Hon, Chung Chau
Zeng, Fanya
Law, Ka Man
Leung, Frederick C.C.
author_facet Chow, Ken Y.C.
Yeung, Yin Shan
Hon, Chung Chau
Zeng, Fanya
Law, Ka Man
Leung, Frederick C.C.
author_sort Chow, Ken Y.C.
collection PubMed
description The pro‐apoptotic properties of severe acute respiratory syndrome coronavirus (SARS‐CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus‐mediated over‐expression of SARS‐CoV spike (S) protein and its C‐terminal domain (S2) induce apoptosis in Vero E6 cells in a time‐ and dosage‐dependent manner, whereas the expression of its N‐terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS‐CoV induced apoptosis and the molecular pathogenesis of SARS.
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spelling pubmed-70944402020-03-25 Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells Chow, Ken Y.C. Yeung, Yin Shan Hon, Chung Chau Zeng, Fanya Law, Ka Man Leung, Frederick C.C. FEBS Lett Short Communications The pro‐apoptotic properties of severe acute respiratory syndrome coronavirus (SARS‐CoV) structural proteins were studied in vitro. By monitoring apoptosis indicators including chromatin condensation, cellular DNA fragmentation and cell membrane asymmetry, we demonstrated that the adenovirus‐mediated over‐expression of SARS‐CoV spike (S) protein and its C‐terminal domain (S2) induce apoptosis in Vero E6 cells in a time‐ and dosage‐dependent manner, whereas the expression of its N‐terminal domain (S1) and other structural proteins, including envelope (E), membrane (M) and nucleocapsid (N) protein do not. These findings suggest a possible role of S and S2 protein in SARS‐CoV induced apoptosis and the molecular pathogenesis of SARS. John Wiley and Sons Inc. 2005-12-19 2005-11-21 /pmc/articles/PMC7094440/ /pubmed/16310778 http://dx.doi.org/10.1016/j.febslet.2005.10.065 Text en FEBS Letters 579 (2005) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Chow, Ken Y.C.
Yeung, Yin Shan
Hon, Chung Chau
Zeng, Fanya
Law, Ka Man
Leung, Frederick C.C.
Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title_full Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title_fullStr Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title_full_unstemmed Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title_short Adenovirus‐mediated expression of the C‐terminal domain of SARS‐CoV spike protein is sufficient to induce apoptosis in Vero E6 cells
title_sort adenovirus‐mediated expression of the c‐terminal domain of sars‐cov spike protein is sufficient to induce apoptosis in vero e6 cells
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094440/
https://www.ncbi.nlm.nih.gov/pubmed/16310778
http://dx.doi.org/10.1016/j.febslet.2005.10.065
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