Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus

SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS...

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Autores principales: Law, Pui Ying Peggy, Liu, Yuet-Man, Geng, Hua, Kwan, Ka Ho, Waye, Mary Miu-Yee, Ho, Yuan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2006
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094570/
https://www.ncbi.nlm.nih.gov/pubmed/16753150
http://dx.doi.org/10.1016/j.febslet.2006.05.051
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author Law, Pui Ying Peggy
Liu, Yuet-Man
Geng, Hua
Kwan, Ka Ho
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
author_facet Law, Pui Ying Peggy
Liu, Yuet-Man
Geng, Hua
Kwan, Ka Ho
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
author_sort Law, Pui Ying Peggy
collection PubMed
description SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in both nucleus and cytoplasm of mammalian cells. Functional study revealed that overexpression of SARS 8b induced DNA synthesis. Coexpression of SARS 8b and SARS 6, a previously characterized SARS‐CoV accessory protein, did not elicit synergistic effects on DNA synthesis.
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spelling pubmed-70945702020-03-25 Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus Law, Pui Ying Peggy Liu, Yuet-Man Geng, Hua Kwan, Ka Ho Waye, Mary Miu-Yee Ho, Yuan-Yuan FEBS Lett Short Communications SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in both nucleus and cytoplasm of mammalian cells. Functional study revealed that overexpression of SARS 8b induced DNA synthesis. Coexpression of SARS 8b and SARS 6, a previously characterized SARS‐CoV accessory protein, did not elicit synergistic effects on DNA synthesis. John Wiley and Sons Inc. 2006-06-26 2006-06-02 /pmc/articles/PMC7094570/ /pubmed/16753150 http://dx.doi.org/10.1016/j.febslet.2006.05.051 Text en FEBS Letters 580 (2006) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Law, Pui Ying Peggy
Liu, Yuet-Man
Geng, Hua
Kwan, Ka Ho
Waye, Mary Miu-Yee
Ho, Yuan-Yuan
Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title_full Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title_fullStr Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title_full_unstemmed Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title_short Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
title_sort expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome‐associated coronavirus
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094570/
https://www.ncbi.nlm.nih.gov/pubmed/16753150
http://dx.doi.org/10.1016/j.febslet.2006.05.051
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