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Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71

Viruses have been known to subvert the anti-apoptotic pathways of the host cell in order to delay apoptosis. However, the mechanisms utilized by enterovirus 71 (EV71) to mediate anti-apoptotic activity remained undetermined. We observed that EV71 infection induced an early activation of both phospha...

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Autores principales: Wong, Wen-Rou, Chen, Yu-Yun, Yang, Shun-Min, Chen, Yin-Li, Horng, Jim-Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094582/
https://www.ncbi.nlm.nih.gov/pubmed/16150462
http://dx.doi.org/10.1016/j.lfs.2005.04.076
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author Wong, Wen-Rou
Chen, Yu-Yun
Yang, Shun-Min
Chen, Yin-Li
Horng, Jim-Tong
author_facet Wong, Wen-Rou
Chen, Yu-Yun
Yang, Shun-Min
Chen, Yin-Li
Horng, Jim-Tong
author_sort Wong, Wen-Rou
collection PubMed
description Viruses have been known to subvert the anti-apoptotic pathways of the host cell in order to delay apoptosis. However, the mechanisms utilized by enterovirus 71 (EV71) to mediate anti-apoptotic activity remained undetermined. We observed that EV71 infection induced an early activation of both phosphatidylinositol 3-kinase (PI3K)/Akt and MAPK/ERK signaling pathways. The activity of GSK3β, a downstream target of these pathways, was negatively regulated by the activation of both MAPK/ERK and PI3K/Akt. The phosphorylation of GSK3 could be inhibited by treatment with the specific inhibitors of MAPK/ERK and PI3K/Akt. Other Akt downstream targets, BAD, caspase-9 and the Forkhead transcription factor (FKHR), were not phosphorylated during the course of infection by EV71. We further demonstrated that infection by UV-irradiated, inactivated virus triggered early Akt activation but was insufficient to trigger late Akt activation. These data suggest that with the phosphorylation of MAPK/ERK and PI3K/Akt the subsequent inactivation of GSK3β is utilized by EV71 as a potential mechanism to delay host cell apoptosis.
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spelling pubmed-70945822020-03-25 Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71 Wong, Wen-Rou Chen, Yu-Yun Yang, Shun-Min Chen, Yin-Li Horng, Jim-Tong Life Sci Article Viruses have been known to subvert the anti-apoptotic pathways of the host cell in order to delay apoptosis. However, the mechanisms utilized by enterovirus 71 (EV71) to mediate anti-apoptotic activity remained undetermined. We observed that EV71 infection induced an early activation of both phosphatidylinositol 3-kinase (PI3K)/Akt and MAPK/ERK signaling pathways. The activity of GSK3β, a downstream target of these pathways, was negatively regulated by the activation of both MAPK/ERK and PI3K/Akt. The phosphorylation of GSK3 could be inhibited by treatment with the specific inhibitors of MAPK/ERK and PI3K/Akt. Other Akt downstream targets, BAD, caspase-9 and the Forkhead transcription factor (FKHR), were not phosphorylated during the course of infection by EV71. We further demonstrated that infection by UV-irradiated, inactivated virus triggered early Akt activation but was insufficient to trigger late Akt activation. These data suggest that with the phosphorylation of MAPK/ERK and PI3K/Akt the subsequent inactivation of GSK3β is utilized by EV71 as a potential mechanism to delay host cell apoptosis. Elsevier Inc. 2005-11-19 2005-09-08 /pmc/articles/PMC7094582/ /pubmed/16150462 http://dx.doi.org/10.1016/j.lfs.2005.04.076 Text en Copyright © 2005 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wong, Wen-Rou
Chen, Yu-Yun
Yang, Shun-Min
Chen, Yin-Li
Horng, Jim-Tong
Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title_full Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title_fullStr Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title_full_unstemmed Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title_short Phosphorylation of PI3K/Akt and MAPK/ERK in an early entry step of enterovirus 71
title_sort phosphorylation of pi3k/akt and mapk/erk in an early entry step of enterovirus 71
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094582/
https://www.ncbi.nlm.nih.gov/pubmed/16150462
http://dx.doi.org/10.1016/j.lfs.2005.04.076
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