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Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein

One of the hallmarks of the human CNS disease subacute sclerosing panencephalitis (SSPE) is a high level of measles virus (MV) infection of oligodendrocytes. It is therefore surprising that there is only one previous report of MV infection of rat oligodendrocytes in culture and no reports of human o...

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Autores principales: Plumb, Jonnie, Duprex, W. Paul, Cameron, C. H. Stewart, Richter-Landsberg, Christiane, Talbot, Pierre, McQuaid, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7095342/
https://www.ncbi.nlm.nih.gov/pubmed/11847589
http://dx.doi.org/10.1080/135502802317247785
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author Plumb, Jonnie
Duprex, W. Paul
Cameron, C. H. Stewart
Richter-Landsberg, Christiane
Talbot, Pierre
McQuaid, Stephen
author_facet Plumb, Jonnie
Duprex, W. Paul
Cameron, C. H. Stewart
Richter-Landsberg, Christiane
Talbot, Pierre
McQuaid, Stephen
author_sort Plumb, Jonnie
collection PubMed
description One of the hallmarks of the human CNS disease subacute sclerosing panencephalitis (SSPE) is a high level of measles virus (MV) infection of oligodendrocytes. It is therefore surprising that there is only one previous report of MV infection of rat oligodendrocytes in culture and no reports of human oligodendrocyte infection in culture. In an attempt to develop a model system to study MV infection of oligodendrocytes, time-lapse confocal microscopy, immunocytochemistry, and electron microscopy (EM) were used to study infection of the human oligodendroglioma cell line, MO3.13. A rat oligodendrocyte cell line, OLN-93, was also studied as a control. MO3.13 cells were shown to be highly susceptible to MV infection and virus budding was observed from the surface of infected MO3.13 cells by EM. Analysis of the infection in real time and by immunocytochemistry revealed that virus spread occurred by cell-to-cell fusion and was also facilitated by virus transport in cell processes. MO3.13 cells were shown to express CD46, a MV receptor, but were negative for the recently discovered MV receptor, signaling leucocyte activation molecule (SLAM). Immunohistochemical studies on SSPE tissue sections demonstrated that CD46 was also expressed on populations of human oligodendrocytes. SLAM expression was not detected on oligodendrocytes. These studies, which are the first to show MV infection of human oligodendrocytes in culture, show that the cells are highly susceptible to MV infection and this model cell line has been used to further our understanding of MV spread in the CNS.
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spelling pubmed-70953422020-03-26 Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein Plumb, Jonnie Duprex, W. Paul Cameron, C. H. Stewart Richter-Landsberg, Christiane Talbot, Pierre McQuaid, Stephen J Neurovirol Article One of the hallmarks of the human CNS disease subacute sclerosing panencephalitis (SSPE) is a high level of measles virus (MV) infection of oligodendrocytes. It is therefore surprising that there is only one previous report of MV infection of rat oligodendrocytes in culture and no reports of human oligodendrocyte infection in culture. In an attempt to develop a model system to study MV infection of oligodendrocytes, time-lapse confocal microscopy, immunocytochemistry, and electron microscopy (EM) were used to study infection of the human oligodendroglioma cell line, MO3.13. A rat oligodendrocyte cell line, OLN-93, was also studied as a control. MO3.13 cells were shown to be highly susceptible to MV infection and virus budding was observed from the surface of infected MO3.13 cells by EM. Analysis of the infection in real time and by immunocytochemistry revealed that virus spread occurred by cell-to-cell fusion and was also facilitated by virus transport in cell processes. MO3.13 cells were shown to express CD46, a MV receptor, but were negative for the recently discovered MV receptor, signaling leucocyte activation molecule (SLAM). Immunohistochemical studies on SSPE tissue sections demonstrated that CD46 was also expressed on populations of human oligodendrocytes. SLAM expression was not detected on oligodendrocytes. These studies, which are the first to show MV infection of human oligodendrocytes in culture, show that the cells are highly susceptible to MV infection and this model cell line has been used to further our understanding of MV spread in the CNS. Springer-Verlag 2002 /pmc/articles/PMC7095342/ /pubmed/11847589 http://dx.doi.org/10.1080/135502802317247785 Text en © Taylor & Francis 2002 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Plumb, Jonnie
Duprex, W. Paul
Cameron, C. H. Stewart
Richter-Landsberg, Christiane
Talbot, Pierre
McQuaid, Stephen
Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title_full Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title_fullStr Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title_full_unstemmed Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title_short Infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
title_sort infection of human oligodendroglioma cells by a recombinant measles virus expressing enhanced green fluorescent protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7095342/
https://www.ncbi.nlm.nih.gov/pubmed/11847589
http://dx.doi.org/10.1080/135502802317247785
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