Developmental plasticity allows outside-in immune responses by resident memory T cells

Central memory T (T(CM)) cells patrol lymph nodes and perform conventional memory responses upon re-stimulation: proliferation, migration, and differentiation into diverse T cell subsets while also self-renewing. Resident memory T (T(RM)) cells are parked within single organs, share properties with terminal effectors, and contribute to rapid host protection. We observed that reactivated T(RM) cells rejoined the circulating pool. Epigenetic analyses revealed that T(RM) cells align closely with conventional memory T cell populations, bearing little resemblance to recently activated effectors. Fully differentiated T(RM) cells isolated from small intestine epithelium exhibited the potential to differentiate into T(CM), T(EM), and T(RM) cells upon recall. Ex-T(RM) cells, former intestinal T(RM) that rejoined the circulating pool, heritably maintained a predilection for homing back to their tissue of origin upon subsequent reactivation and a heightened capacity to re-differentiate into T(RM) cells. Thus, T(RM) cells can rejoin the circulation but are advantaged to re-form local T(RM) when called upon.