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Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technolog...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096552/ https://www.ncbi.nlm.nih.gov/pubmed/32266291 http://dx.doi.org/10.3389/fcvm.2020.00031 |
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author | Diallo, Idrissa Benmoussa, Abderrahim Laugier, Jonathan Osman, Abdimajid Hitzler, Walter E. Provost, Patrick |
author_facet | Diallo, Idrissa Benmoussa, Abderrahim Laugier, Jonathan Osman, Abdimajid Hitzler, Walter E. Provost, Patrick |
author_sort | Diallo, Idrissa |
collection | PubMed |
description | Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technologies (PRTs), such as Intercept and Mirasol, utilize amotosalen, and riboflavin (vitamin B2), respectively, to mediate inactivation of pathogen nucleic acids. Although they are expected to increase the safety and prolong the shelf life of clinical PCs, these PRTs might affect the quality and function of platelets, as recently reported. Upon activation, platelets release microparticles (MPs), which are involved in intercellular communications and regulation of gene expression, thereby mediating critical cellular functions. Here, we have used small RNA sequencing (RNA-Seq) to document the effect of PRT treatment on the microRNA profiles of platelets and derived MPs. PRT treatment did not affect the microRNA profile of platelets. However, we observed a specific loading of certain microRNAs into platelet MPs, which was impaired by treatment with Intercept or its Additive solution (SSP+). Whereas, Intercept had an impact on the microRNA profile of platelet-derived MPs, Mirasol did not impact the microRNA profile of platelets and derived MPs, compared to non-treated control. Considering that platelet MPs are able to transfer their microRNA content to recipient cells, and that this content may exert biological activities, those findings suggest that PRT treatment of clinical PCs may modify the bioactivity of the platelets and MPs to be transfused and argue for further investigations into PRT-induced changes in clinical PC content and function. |
format | Online Article Text |
id | pubmed-7096552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70965522020-04-07 Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles Diallo, Idrissa Benmoussa, Abderrahim Laugier, Jonathan Osman, Abdimajid Hitzler, Walter E. Provost, Patrick Front Cardiovasc Med Cardiovascular Medicine Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technologies (PRTs), such as Intercept and Mirasol, utilize amotosalen, and riboflavin (vitamin B2), respectively, to mediate inactivation of pathogen nucleic acids. Although they are expected to increase the safety and prolong the shelf life of clinical PCs, these PRTs might affect the quality and function of platelets, as recently reported. Upon activation, platelets release microparticles (MPs), which are involved in intercellular communications and regulation of gene expression, thereby mediating critical cellular functions. Here, we have used small RNA sequencing (RNA-Seq) to document the effect of PRT treatment on the microRNA profiles of platelets and derived MPs. PRT treatment did not affect the microRNA profile of platelets. However, we observed a specific loading of certain microRNAs into platelet MPs, which was impaired by treatment with Intercept or its Additive solution (SSP+). Whereas, Intercept had an impact on the microRNA profile of platelet-derived MPs, Mirasol did not impact the microRNA profile of platelets and derived MPs, compared to non-treated control. Considering that platelet MPs are able to transfer their microRNA content to recipient cells, and that this content may exert biological activities, those findings suggest that PRT treatment of clinical PCs may modify the bioactivity of the platelets and MPs to be transfused and argue for further investigations into PRT-induced changes in clinical PC content and function. Frontiers Media S.A. 2020-03-19 /pmc/articles/PMC7096552/ /pubmed/32266291 http://dx.doi.org/10.3389/fcvm.2020.00031 Text en Copyright © 2020 Diallo, Benmoussa, Laugier, Osman, Hitzler and Provost. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Diallo, Idrissa Benmoussa, Abderrahim Laugier, Jonathan Osman, Abdimajid Hitzler, Walter E. Provost, Patrick Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title | Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title_full | Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title_fullStr | Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title_full_unstemmed | Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title_short | Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles |
title_sort | platelet pathogen reduction technologies alter the microrna profile of platelet-derived microparticles |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096552/ https://www.ncbi.nlm.nih.gov/pubmed/32266291 http://dx.doi.org/10.3389/fcvm.2020.00031 |
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