Cargando…

Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles

Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technolog...

Descripción completa

Detalles Bibliográficos
Autores principales: Diallo, Idrissa, Benmoussa, Abderrahim, Laugier, Jonathan, Osman, Abdimajid, Hitzler, Walter E., Provost, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096552/
https://www.ncbi.nlm.nih.gov/pubmed/32266291
http://dx.doi.org/10.3389/fcvm.2020.00031
_version_ 1783510832627318784
author Diallo, Idrissa
Benmoussa, Abderrahim
Laugier, Jonathan
Osman, Abdimajid
Hitzler, Walter E.
Provost, Patrick
author_facet Diallo, Idrissa
Benmoussa, Abderrahim
Laugier, Jonathan
Osman, Abdimajid
Hitzler, Walter E.
Provost, Patrick
author_sort Diallo, Idrissa
collection PubMed
description Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technologies (PRTs), such as Intercept and Mirasol, utilize amotosalen, and riboflavin (vitamin B2), respectively, to mediate inactivation of pathogen nucleic acids. Although they are expected to increase the safety and prolong the shelf life of clinical PCs, these PRTs might affect the quality and function of platelets, as recently reported. Upon activation, platelets release microparticles (MPs), which are involved in intercellular communications and regulation of gene expression, thereby mediating critical cellular functions. Here, we have used small RNA sequencing (RNA-Seq) to document the effect of PRT treatment on the microRNA profiles of platelets and derived MPs. PRT treatment did not affect the microRNA profile of platelets. However, we observed a specific loading of certain microRNAs into platelet MPs, which was impaired by treatment with Intercept or its Additive solution (SSP+). Whereas, Intercept had an impact on the microRNA profile of platelet-derived MPs, Mirasol did not impact the microRNA profile of platelets and derived MPs, compared to non-treated control. Considering that platelet MPs are able to transfer their microRNA content to recipient cells, and that this content may exert biological activities, those findings suggest that PRT treatment of clinical PCs may modify the bioactivity of the platelets and MPs to be transfused and argue for further investigations into PRT-induced changes in clinical PC content and function.
format Online
Article
Text
id pubmed-7096552
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-70965522020-04-07 Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles Diallo, Idrissa Benmoussa, Abderrahim Laugier, Jonathan Osman, Abdimajid Hitzler, Walter E. Provost, Patrick Front Cardiovasc Med Cardiovascular Medicine Despite improvements in donor screening and increasing efforts to avoid contamination and the spread of pathogens in clinical platelet concentrates (PCs), the risks of transfusion-transmitted infections remain important. Relying on an ultraviolet photo activation system, pathogen reduction technologies (PRTs), such as Intercept and Mirasol, utilize amotosalen, and riboflavin (vitamin B2), respectively, to mediate inactivation of pathogen nucleic acids. Although they are expected to increase the safety and prolong the shelf life of clinical PCs, these PRTs might affect the quality and function of platelets, as recently reported. Upon activation, platelets release microparticles (MPs), which are involved in intercellular communications and regulation of gene expression, thereby mediating critical cellular functions. Here, we have used small RNA sequencing (RNA-Seq) to document the effect of PRT treatment on the microRNA profiles of platelets and derived MPs. PRT treatment did not affect the microRNA profile of platelets. However, we observed a specific loading of certain microRNAs into platelet MPs, which was impaired by treatment with Intercept or its Additive solution (SSP+). Whereas, Intercept had an impact on the microRNA profile of platelet-derived MPs, Mirasol did not impact the microRNA profile of platelets and derived MPs, compared to non-treated control. Considering that platelet MPs are able to transfer their microRNA content to recipient cells, and that this content may exert biological activities, those findings suggest that PRT treatment of clinical PCs may modify the bioactivity of the platelets and MPs to be transfused and argue for further investigations into PRT-induced changes in clinical PC content and function. Frontiers Media S.A. 2020-03-19 /pmc/articles/PMC7096552/ /pubmed/32266291 http://dx.doi.org/10.3389/fcvm.2020.00031 Text en Copyright © 2020 Diallo, Benmoussa, Laugier, Osman, Hitzler and Provost. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Diallo, Idrissa
Benmoussa, Abderrahim
Laugier, Jonathan
Osman, Abdimajid
Hitzler, Walter E.
Provost, Patrick
Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title_full Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title_fullStr Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title_full_unstemmed Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title_short Platelet Pathogen Reduction Technologies Alter the MicroRNA Profile of Platelet-Derived Microparticles
title_sort platelet pathogen reduction technologies alter the microrna profile of platelet-derived microparticles
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096552/
https://www.ncbi.nlm.nih.gov/pubmed/32266291
http://dx.doi.org/10.3389/fcvm.2020.00031
work_keys_str_mv AT dialloidrissa plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles
AT benmoussaabderrahim plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles
AT laugierjonathan plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles
AT osmanabdimajid plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles
AT hitzlerwaltere plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles
AT provostpatrick plateletpathogenreductiontechnologiesalterthemicrornaprofileofplateletderivedmicroparticles