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Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions

Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural...

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Autores principales: Eguchi, Kohgaku, Velicky, Philipp, Hollergschwandtner, Elena, Itakura, Makoto, Fukazawa, Yugo, Danzl, Johann Georg, Shigemoto, Ryuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096554/
https://www.ncbi.nlm.nih.gov/pubmed/32265664
http://dx.doi.org/10.3389/fncel.2020.00063
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author Eguchi, Kohgaku
Velicky, Philipp
Hollergschwandtner, Elena
Itakura, Makoto
Fukazawa, Yugo
Danzl, Johann Georg
Shigemoto, Ryuichi
author_facet Eguchi, Kohgaku
Velicky, Philipp
Hollergschwandtner, Elena
Itakura, Makoto
Fukazawa, Yugo
Danzl, Johann Georg
Shigemoto, Ryuichi
author_sort Eguchi, Kohgaku
collection PubMed
description Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions.
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spelling pubmed-70965542020-04-07 Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions Eguchi, Kohgaku Velicky, Philipp Hollergschwandtner, Elena Itakura, Makoto Fukazawa, Yugo Danzl, Johann Georg Shigemoto, Ryuichi Front Cell Neurosci Cellular Neuroscience Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions. Frontiers Media S.A. 2020-03-19 /pmc/articles/PMC7096554/ /pubmed/32265664 http://dx.doi.org/10.3389/fncel.2020.00063 Text en Copyright © 2020 Eguchi, Velicky, Hollergschwandtner, Itakura, Fukazawa, Danzl and Shigemoto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Eguchi, Kohgaku
Velicky, Philipp
Hollergschwandtner, Elena
Itakura, Makoto
Fukazawa, Yugo
Danzl, Johann Georg
Shigemoto, Ryuichi
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title_full Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title_fullStr Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title_full_unstemmed Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title_short Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
title_sort advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096554/
https://www.ncbi.nlm.nih.gov/pubmed/32265664
http://dx.doi.org/10.3389/fncel.2020.00063
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