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Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum

Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This pape...

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Detalles Bibliográficos
Autores principales: Oda, Hiroki, Akiyama-Oda, Yasuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096682/
https://www.ncbi.nlm.nih.gov/pubmed/32226819
http://dx.doi.org/10.1016/j.dib.2019.105088
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author Oda, Hiroki
Akiyama-Oda, Yasuko
author_facet Oda, Hiroki
Akiyama-Oda, Yasuko
author_sort Oda, Hiroki
collection PubMed
description Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embryonic patterning in spider embryos.
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spelling pubmed-70966822020-03-27 Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum Oda, Hiroki Akiyama-Oda, Yasuko Data Brief Genetics, Genomics and Molecular Biology Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embryonic patterning in spider embryos. Elsevier 2020-01-03 /pmc/articles/PMC7096682/ /pubmed/32226819 http://dx.doi.org/10.1016/j.dib.2019.105088 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Genetics, Genomics and Molecular Biology
Oda, Hiroki
Akiyama-Oda, Yasuko
Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title_full Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title_fullStr Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title_full_unstemmed Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title_short Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
title_sort dataset on gene expressions affected by simultaneous knockdown of hedgehog and dpp signaling components in embryos of the spider parasteatoda tepidariorum
topic Genetics, Genomics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096682/
https://www.ncbi.nlm.nih.gov/pubmed/32226819
http://dx.doi.org/10.1016/j.dib.2019.105088
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