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Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This pape...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096682/ https://www.ncbi.nlm.nih.gov/pubmed/32226819 http://dx.doi.org/10.1016/j.dib.2019.105088 |
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author | Oda, Hiroki Akiyama-Oda, Yasuko |
author_facet | Oda, Hiroki Akiyama-Oda, Yasuko |
author_sort | Oda, Hiroki |
collection | PubMed |
description | Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embryonic patterning in spider embryos. |
format | Online Article Text |
id | pubmed-7096682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70966822020-03-27 Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum Oda, Hiroki Akiyama-Oda, Yasuko Data Brief Genetics, Genomics and Molecular Biology Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-color microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embryonic patterning in spider embryos. Elsevier 2020-01-03 /pmc/articles/PMC7096682/ /pubmed/32226819 http://dx.doi.org/10.1016/j.dib.2019.105088 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Genetics, Genomics and Molecular Biology Oda, Hiroki Akiyama-Oda, Yasuko Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title | Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title_full | Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title_fullStr | Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title_full_unstemmed | Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title_short | Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
title_sort | dataset on gene expressions affected by simultaneous knockdown of hedgehog and dpp signaling components in embryos of the spider parasteatoda tepidariorum |
topic | Genetics, Genomics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096682/ https://www.ncbi.nlm.nih.gov/pubmed/32226819 http://dx.doi.org/10.1016/j.dib.2019.105088 |
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