Cargando…

Rolling circle amplification as isothermal gene amplification in molecular diagnostics

Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. Th...

Descripción completa

Detalles Bibliográficos
Autores principales: Goo, Nam-In, Kim, Dong-Eun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean BioChip Society (KBCS) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096790/
https://www.ncbi.nlm.nih.gov/pubmed/32226587
http://dx.doi.org/10.1007/s13206-016-0402-6
_version_ 1783510910782930944
author Goo, Nam-In
Kim, Dong-Eun
author_facet Goo, Nam-In
Kim, Dong-Eun
author_sort Goo, Nam-In
collection PubMed
description Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations.
format Online
Article
Text
id pubmed-7096790
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Korean BioChip Society (KBCS)
record_format MEDLINE/PubMed
spelling pubmed-70967902020-03-26 Rolling circle amplification as isothermal gene amplification in molecular diagnostics Goo, Nam-In Kim, Dong-Eun Biochip J Review Article Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations. The Korean BioChip Society (KBCS) 2016-07-29 2016 /pmc/articles/PMC7096790/ /pubmed/32226587 http://dx.doi.org/10.1007/s13206-016-0402-6 Text en © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Review Article
Goo, Nam-In
Kim, Dong-Eun
Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title_full Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title_fullStr Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title_full_unstemmed Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title_short Rolling circle amplification as isothermal gene amplification in molecular diagnostics
title_sort rolling circle amplification as isothermal gene amplification in molecular diagnostics
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096790/
https://www.ncbi.nlm.nih.gov/pubmed/32226587
http://dx.doi.org/10.1007/s13206-016-0402-6
work_keys_str_mv AT goonamin rollingcircleamplificationasisothermalgeneamplificationinmoleculardiagnostics
AT kimdongeun rollingcircleamplificationasisothermalgeneamplificationinmoleculardiagnostics