Cargando…
Rolling circle amplification as isothermal gene amplification in molecular diagnostics
Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. Th...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean BioChip Society (KBCS)
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096790/ https://www.ncbi.nlm.nih.gov/pubmed/32226587 http://dx.doi.org/10.1007/s13206-016-0402-6 |
_version_ | 1783510910782930944 |
---|---|
author | Goo, Nam-In Kim, Dong-Eun |
author_facet | Goo, Nam-In Kim, Dong-Eun |
author_sort | Goo, Nam-In |
collection | PubMed |
description | Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations. |
format | Online Article Text |
id | pubmed-7096790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean BioChip Society (KBCS) |
record_format | MEDLINE/PubMed |
spelling | pubmed-70967902020-03-26 Rolling circle amplification as isothermal gene amplification in molecular diagnostics Goo, Nam-In Kim, Dong-Eun Biochip J Review Article Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations. The Korean BioChip Society (KBCS) 2016-07-29 2016 /pmc/articles/PMC7096790/ /pubmed/32226587 http://dx.doi.org/10.1007/s13206-016-0402-6 Text en © The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Article Goo, Nam-In Kim, Dong-Eun Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title | Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title_full | Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title_fullStr | Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title_full_unstemmed | Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title_short | Rolling circle amplification as isothermal gene amplification in molecular diagnostics |
title_sort | rolling circle amplification as isothermal gene amplification in molecular diagnostics |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096790/ https://www.ncbi.nlm.nih.gov/pubmed/32226587 http://dx.doi.org/10.1007/s13206-016-0402-6 |
work_keys_str_mv | AT goonamin rollingcircleamplificationasisothermalgeneamplificationinmoleculardiagnostics AT kimdongeun rollingcircleamplificationasisothermalgeneamplificationinmoleculardiagnostics |