miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer

Gastric cancer (GC) exhibits a poor prognosis due to extensive invasion and lymphatic metastasis in the advanced stage. In this study, we firstly found that the expression of miR-204-5p markedly decreased in GC patients' tissue and serum, especially in GC with lymphatic metastasis. And ROC anal...

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Autores principales: Zhang, Juan, Xing, Ling, Xu, Hongwei, Wang, Kaixuan, She, Junjun, Shi, Feiyu, Wu, Hongyu, Sun, Yongjie, Gao, Jun, He, Shuixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097947/
https://www.ncbi.nlm.nih.gov/pubmed/32231725
http://dx.doi.org/10.7150/jca.33273
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author Zhang, Juan
Xing, Ling
Xu, Hongwei
Wang, Kaixuan
She, Junjun
Shi, Feiyu
Wu, Hongyu
Sun, Yongjie
Gao, Jun
He, Shuixiang
author_facet Zhang, Juan
Xing, Ling
Xu, Hongwei
Wang, Kaixuan
She, Junjun
Shi, Feiyu
Wu, Hongyu
Sun, Yongjie
Gao, Jun
He, Shuixiang
author_sort Zhang, Juan
collection PubMed
description Gastric cancer (GC) exhibits a poor prognosis due to extensive invasion and lymphatic metastasis in the advanced stage. In this study, we firstly found that the expression of miR-204-5p markedly decreased in GC patients' tissue and serum, especially in GC with lymphatic metastasis. And ROC analysis showed miR-204-5p also served as a predicted factor for the lymphatic metastasis of GC. CXCL12 and CXCR4 were predicted and confirmed as the functional targets of miR-204-5p by Targetscan analysis, dual luciferase assay and western blotting analysis. In addition, we further determined that miR-204-5p suppresses migration and invasion in GC. This finding elucidates new functions and mechanisms for miR-204-5p in GC development and provides a new potential diagnostic marker and therapeutic targets for GC.
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spelling pubmed-70979472020-03-30 miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer Zhang, Juan Xing, Ling Xu, Hongwei Wang, Kaixuan She, Junjun Shi, Feiyu Wu, Hongyu Sun, Yongjie Gao, Jun He, Shuixiang J Cancer Research Paper Gastric cancer (GC) exhibits a poor prognosis due to extensive invasion and lymphatic metastasis in the advanced stage. In this study, we firstly found that the expression of miR-204-5p markedly decreased in GC patients' tissue and serum, especially in GC with lymphatic metastasis. And ROC analysis showed miR-204-5p also served as a predicted factor for the lymphatic metastasis of GC. CXCL12 and CXCR4 were predicted and confirmed as the functional targets of miR-204-5p by Targetscan analysis, dual luciferase assay and western blotting analysis. In addition, we further determined that miR-204-5p suppresses migration and invasion in GC. This finding elucidates new functions and mechanisms for miR-204-5p in GC development and provides a new potential diagnostic marker and therapeutic targets for GC. Ivyspring International Publisher 2020-03-05 /pmc/articles/PMC7097947/ /pubmed/32231725 http://dx.doi.org/10.7150/jca.33273 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Zhang, Juan
Xing, Ling
Xu, Hongwei
Wang, Kaixuan
She, Junjun
Shi, Feiyu
Wu, Hongyu
Sun, Yongjie
Gao, Jun
He, Shuixiang
miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title_full miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title_fullStr miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title_full_unstemmed miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title_short miR-204-5p Suppress Lymph Node Metastasis via Regulating CXCL12 and CXCR4 in Gastric Cancer
title_sort mir-204-5p suppress lymph node metastasis via regulating cxcl12 and cxcr4 in gastric cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097947/
https://www.ncbi.nlm.nih.gov/pubmed/32231725
http://dx.doi.org/10.7150/jca.33273
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